Izvestiya of Saratov University.
ISSN 1816-9775 (Print)
ISSN 2541-8971 (Online)


Биология

Obtaining a fluorescently labeled endophytic strain of bacteria Herbaspirillum lusitanum P6-12 for their detection in vivo and in vitro

The Herbaspirillum lusitanum P6-12 strain containing the vector plasmid pJN105TurboGFP, which encodes the synthesis of the green fluorescent protein GFP, and which has resistance to the antibiotic gentamicin, was obtained by electroporation. The constructed strain of H. lusitanum P6-12 in cultural, morphological and biochemical properties did not differ from the original typical natural strain of H. lusitanum P6-12. On solid growth media, the recombinant strain formed yellow-green colonies, fluorescent under UV irradiation.

Faunistic complex of mycetophilous beetles (Insecta: Coleoptera) of the xylotrophic fungus Fomes fomentarius (Basidiomyceta) in the territory of the Saratov region

This article describes the characteristics of the beetle fauna – inhabitants of fruiting bodies and the mycelial layer of the true tinder fungus Fomes fomentarius L.: Fr. and are considers some important aspects of the ecology of mycetophilous beetles. Coleopteran population of fruiting bodies of the fungus in five districts of the Saratov region is 63%. Two species from the family Staphylinidae (Gyrophaena joyi Wendeler, 1924 and Scaphisoma boreale Lundblad, 1952) have been recorded on growing and / or spore bearing fruiting bodies.

Evaluation of the secondary structure of poly-?-glutamic acid produced by Bacillus subtilis EGP5QL12 by circular dichroism spectroscopy method

An extracellular polymer was isolated from the culture liquid of Bacillus subtilis EGP5QL12 with the yield of 5,6 g/L. On the basis of the data of thin layer chromatography, colorimetric analyses and FTIR spectroscopy, it was established that the polymer is poly-?glutamic acid (PGA). PGA is widely used in medicine, cosmetology and the food industry due to its ability to bind water and metal ions.

Stress signals of water scavenger beetles Berosus frontifoveatus Kuwert, 1888 and Berosus spinosus Steven, 1808 (Coleoptera: Hydrophilidae)

The study of acoustic signals from various representatives of insects, and in particular coleopterans, has a long history. In the Russian-language literature, systematic, faunistic and ecological studies on aquatic beetles, including those on the family Hydrophilidae, are widely known; however, studies on the bioacoustics of the coleopteran group are quite rare. The aim of our study was to study stress signals of two species of Hydrophilidae – Berosus frontifoveatus Kuwert, 1888 and Berosus spinosus Steven, 1808.

Features of selection of winter wheat on grain quality

The quality of grain means a set of technologico-biochemical, baking and food properties determining the economic value of a grain variety. When choosing source material for selection, the degree of grain preservation in adverse environmental conditions is taken into account as well as the genotypic potential of grain yield and quality.

Winter months’ temperature rises effect on duration of phenological phases for Spermophilus pygmaeus Pallas, 1778 populations located in Caspian depression (Astrakhan oblast)

This article provides comparison analysis between 1950–1959’s and 2000–2009’s periods for duration and schedule dates of awakening, spreading and hibernation phases for Spermophilus pygmaeus populations located in the Caspian Depression (by example of Astrakhan Oblast). We learned that within a period of maximum temperature rise in February 2000–2009’s durations of S.pygmaeus’ populations of every phonological phase have shortened and been rescheduled with earlier dates (in comparison to 1950–1959’s).

Antibacterial properties of a biologically active composite based on glauconite

Glauconite, a natural aluminosilicate, exhibits good sorption properties, is an affordable, cheap material, and has the prospect of being used in construction, ecology, medicine, cosmetology, animal husbandry, poultry farming, agriculture, etc. An urgent task is to study the sorption activity of glauconite in the Beloozersky deposit in the Saratov region in relation to biologically active substances for the creation of antimicrobial composites. The elemental composition and surface morphology of glauconite grains are determined.

Introduction of sorts of Clematis L. genus in the South-Ural Botanical Garden-Institute of UFRC RAS

The article presents the results of a long-term of study of biological features of 54 sorts of Clematis L. genus of collection of the SouthUral Botanical Garden-Institute of UFRC RAS. The aim of the work was to summarize the introduction studies for the possibility of successful use of clematis sorts in vertical gardening in the Bashkir Cis-Urals and adjacent territories.

Development of female hametophytis and embryogenesis of introduced species Picea A. Dietr. in the Taiga Zone (Karelia)

The aim of the research was to establish features in macrosporogenesis, macrogametogenesis, and embryogenesis in Picea species (native species P. abies and introduced species P. canadensis and P. pungens) in the Botanical Garden of Petrozavodsk State University (middle taiga subzone). To study the development of the generative sphere, shoots with generative buds and strobiles from the southwestern side of the crown were cut from 10 trees and fixed in a mixture of alcohol and glycerin.

Chlorophyllin Inhibits Chemiluminescence That Accompanies a Quasi-Hypoxygenase Reaction Catalyzed by the Cytochrome c-Cardiolipin Complex

Lipid peroxidation is a key factor in programmed cell death of various etiologies. This process also occurs when ionizing radiation acts on biological systems. It is the essence of the theory of lipid radiotoxins, which is part of the structural and metabolic theory of the biological action of ionizing radiation. Non-enzymatic lipid peroxidation eventually leads to death by the mechanism of ferroptosis, while enzymatic lipid peroxidation, catalyzed by the cytochrome c-cardiolipin complex, triggers apoptosis along the mitochondrial pathway.

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