Биология

Large-scale production and use of antimicrobials in human, veterinary and agricultural applications has led to antibiotic contamination of water resources. Therefore, it is relevant to develop methods for monitoring the content of antibiotics, especially in water resources. Biosensor methods are successful for the analysis of antibiotics. One of the main elements of a biosensor system is the selection of a recognition element.

In this work, we studied the development of tolerance to low-intensity violet (405 nm, 80 mW/cm2 , 72 J/cm2 ) LED radiation in a clinical antibiotic-resistant strain of Staphylococcus aureus 2a. The change in numbers during 20 cycles of irradiation was studied, the reaction of bacterial cells to oxidative stress was studied – sensitivity to the presence of hydrogen peroxide in the environment and catalase activity.

Phospholipids are the most important structural elements of the bacterial cell wall, participate in the adaptation of microorganisms to the environment and can act as biomarkers for environmental changes and one of the components of environmental monitoring. Native phospholipids are used to form models of cell membranes, the biophysical properties of which can be studied by the Langmuir-Blodgett method.

Dihaploid lines, or lines obtained on the basis of doubled haploids, are a valuable material for the creation of high-heterosis corn hybrids. The most eff ective method of evaluating the starting material when selecting parental couples for obtaining hybrids is diallell analysis. The article presents the results of the analysis of indicators of 6 dihaploid corn lines and 30 simple corn hybrids of the dialle scheme. The combinative ability of lines along the stem length, the cob attachment height, and the panicle length was studied.

Concerning the distribution of Allium caeruleum Pall. and A. regelianum A. K. Becker, recent literary sources provide confl icting information. Based on the data from the doctoral dissertation of A. O. Tarasov, the results of expeditionary research and our own herbarium collections, it is stated that Allium caeruleum is distributed in the Ozinsky (at least in the vicinity of the village of Nepryakin) and in the Aleksandrovo-Gaisky (at least in the vicinity of the village of Kamyshki) districts of the Saratov region. The presence of A.

Multicellular spheroids are three-dimensional in vitro models of organs and tissues. Multicellular spheroids have gained great interest in the fi eld of biotechnology as they are reproducible and mimic real organs and tissues so can be used as test systems for new forms of drugs, allowing minimizing the use of in vivo animal models. Flow cytometry was used to describe the accumulation of submicron polymeric microcapsules in cellular spheroids, which allowed us to identify the main aspects of drug carrier-cell interactions within the spheroid.