Izvestiya of Saratov University.

Chemistry. Biology. Ecology

ISSN 1816-9775 (Print)
ISSN 2541-8971 (Online)

For citation:

Revin V. V., Parchaykina M. V., Chudaikina E. V., Revina E. S., Molchanov I. D., Simakova M. A., Zavarykina A. V., Grunyushkin I. P., Devyatkin A. А. Study of the influence of various physiologically active substances on changes in the lipid composition and phospholipase activity of damaged somatic nerves. Izvestiya of Saratov University. Chemistry. Biology. Ecology, 2024, vol. 24, iss. 4, pp. 448-460. DOI: 10.18500/1816-9775-2024-24-4-448-460, EDN: QJXELD

This is an open access article distributed under the terms of Creative Commons Attribution 4.0 International License (CC-BY 4.0).
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Language: 
Russian
Heading: 
Biology
Article type: 
Article
UDC: 
57:612.01:577.115
DOI: 
10.18500/1816-9775-2024-24-4-448-460
EDN: 
QJXELD

Study of the influence of various physiologically active substances on changes in the lipid composition and phospholipase activity of damaged somatic nerves

Autors: 
Revin Viktor V., Ogarev Mordovia State University
Parchaykina Marina V., Ogarev Mordovia State University
Chudaikina Elena V., Ogarev Mordovia State University
Revina Elvira S., Ogarev Mordovia State University
Molchanov Ivan D., Ogarev Mordovia State University
Simakova Milena A., Ogarev Mordovia State University
Zavarykina Anastasia Vyacheslavovna, Ogarev Mordovia State University
Grunyushkin Igor P., Ogarev Mordovia State University
Devyatkin Arkadiy А., Ogarev Mordovia State University
Abstract: 

The changes in lipid composition and phospholipase activity in damaged somatic nerves were studied against the background of the action of potassium hyaluronate and insulin-like growth factor-1. It has been shown that nerve cutting is accompanied by an increase in phospholipase A2 activity, resulting in an accumulation of lysophospholipids and free fatty acids, as well as an increase in phosphatidylinositol levels and a decrease in diacylglycerol content, which is most likely due to inactivation of phosphoinositide-specifi c phospholipase C against the background of injury to the nerve conductor. The introduction of potassium hyaluronate and insulin-like growth factor-1 enhances the recovery processes in the injured nerve conductor, however, the mechanisms of their action remain diff erent. According to the literature data and the results of our own research, the action of potassium hyaluronate and insulin-like growth factor-1 is realized as a result of the launch of signaling pathways associated with the regulation of the activity of enzymes from the phospholipase family. At the same time, our data on a decrease in the activity of phospholipase A2 and the absence of signifi cant changes in the level of phosphatidylinositol and diacylglycerol indicate that potassium hyaluronate most likely exerts its eff ect through the PL A2 -mediated pathway. In addition, it was shown that against the background of the action of IGF-1, an intensifi cation of phosphoinositide metabolism is observed, which is explained by the activation of phosphoinositidespecifi c phospholipase C. According to the literature, the launch of the phospholipase C-mediated mechanism is accompanied by the formation of components of the phosphatidylinositol 3-kinase signaling pathway involved in stimulation of the expression of various transcription factors necessary for axonal regeneration and restoration of the functioning of injured nerve conductors.

Key words: 
lysophospholipids
free fatty acids
potassium hyaluronate
insulin-like growth factor-1
regeneration
somatic nerves
phospholipase activity
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Received: 
30.08.2024
Accepted: 
20.09.2024
Published: 
25.12.2024
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