Screening of Genus Azospirillum for their Ability to Produce Extracellular Lignin-Peroxidase and the Degradation of Model Lignin Compounds and Azo Dyes
Lignin peroxidase is one of the main enzymes of fungi decomposers of wood, that is capable to many non-specific oxidation of aromatic and polycyclic compounds. By the beginning of our research there were virtually no data of the bacteria’s ability to produce the lignin peroxidase. Rather recently lignin peroxidase activity was detected by us in the washouts from the surface of the bacterial cells and in intracellular extracts of bacteria from genus Azospirillum. In this study, 6 strains of bacteria of the genus Azospirillumwas screened by their ability to produce extracellular lignin peroxidase, and by the potential of these microorganisms to the degradation of lignin model compounds and azo dyes. The enzyme activity in the culture fluid was determined by oxidation of veratryl alcohol to veratric aldehyde. Lignin-degrading capacity of the bacteria was determined by the method of Ahmad, using preparations of nitrided lignin. In the study of the bacteria of the genus Azospirillum by their ability to degrading the synthetic dyes, the methyl orange was selected as a model of the azo dye. The study found production of extracellular lignin peroxidase from all strains of bacteria of the genus Azospirillum taken in the experiment. As a result of screening revealed Azospirillum ability to degradation of lignin model compounds. For the first time discovered the ability of bacteria of the genus Azospirillum to the destruction of azo dyes. In most cases showed positive correlation between the level of activity of extracellular lignin peroxidase and ability to degradation of lignin model compounds and aromatic complex dyes.
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