Биология

We optimizes the composition and concentration of the enzymes, the time for enzymatic treatment and the volume of the enzyme mixture. We also optimized the concentration osmotic of agent, the centrifugation mode, and fi lter pore size for protoplasts isolating from epidermal cells of maize roots (Zea mays L.) of the Brown Marker (BM) line. It was found that 150 minutes is the optimal time for 150 mg root tissue maceration.

The paper presents the results of the study of the biological features of Orthosiphon aristatus (Blume) Miq. (awned orthosiphon) in open ground conditions on the Southern Coast of the Crimea and in a greenhouse. Features of fl owering, structure of male and female generative structures are revealed.

. Recently, much attention has been paid to the development of technologies for biodegradation of organopollutants and the search for promising biodestructors. The environmental accumulation of lignin-like compounds and synthetic dyes poses a huge threat not only to ecosystems and biodiversity, but also to human health. Phenol oxidases are enzymes with broad substrate specifi city, with oxidizing ability towards various polyphenols and aromatic amines. Therefore the use of phenol oxydases as bioremediation agents is promising due to their unique catalytic properties.

The article for the fi rst time presents the comparative results of the content of ash substances, sulfur, phenolic compounds (tannins), ascorbic acid in the leaves of plants Iris hybrida, Hosta decorata, H. albomarginata, H. lancifolia, cultivated in the Central Siberian Botanical Garden (CSBS, Akademgorodok) and used in landscaping the urban environment of the Berdsk and Koltsovo settlements (Novosibirsk region) during the seasonal development in 2020.

From the stand point of the possible use of microorganisms in water purifi cation biotechnologies, studying microorganisms capable of removing dangerous pollutants such as heavy metals from the aquatic environment is of great importance. Microbial screening was conducted on isolates from microbiocenoses of highly magnetic soils of the city Mednogorsk (Orenburg region, Russia), to assess their resistance to manganese (II) ions.

Crambid moth Neocrambus wolfschlaegeri (Schawerda, 1937) is reported from the Volga-Ural Region (Orenburg and Saratov Provinces). This represents the fi rst record of this species from Russia. The external morphology and genitalia are illustrated and some details of the morphology are discussed.

. The article presents data on the new location of scorpions of the genus Mesobuthus in the Orenburg Nature Reserve. The aim of the work is to determine the species on the basis of the identifi ed morphological features of populations living in the Southern Urals (the territory of the Orenburg Nature Reserve, Orenburg region, Kuvandyksky Mountains.

The features of the development of safflower (Carthamus tinctorius L.) in regions that are significantly remote geographically and differ sharply in environmental and climatic conditions – Central Asia (Republic of Tajikistan, Sughd region; Republic of Kazakhstan, Zhambyl region) and the Central Black Earth region of the Russian Federation (Voronezh region). A comparative analysis of the development of C.