Izvestiya of Saratov University.

Chemistry. Biology. Ecology

ISSN 1816-9775 (Print)
ISSN 2541-8971 (Online)

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[57.063.8:616.98:579.861.2] – 048.88:615.373.35:615.849.19 (045)

Photodynamic Effect of Red Laser Radiation on the Growth of Staphylococcus aureus Strains with the Use of Photoditazine

Egorova А. V., Saratov State Medical University
Brill G. E., Saratov State Medical University
Bugaeva I. O., Saratov State Medical University
Tuchina Elena S., Saratov State University
Nechayeva O V, Saratov State Medical University

The influence of red laser irradiation on the growth of colonies of Staphylococcus aureus and photodynamic effect of the photosensi- tizer Photoditazin were performed. It is established that the radiation of a semiconductor red laser (? = 660 nm, 100 mW/cm2.) has a direct bacteriostatic effect on the growth of S. aureus. This effect on the standard strain is manifested only when relatively high doses are used (180 J/cm2). Inhibition of bacterial growth reaches 36%. Photosensitivity of the methicillin-resistant strains was much higher: bacteriostatic effect of red light was observed already at the dose of 60 J/cm2. At the maximum dose of irradiation used in our experi- ments, the inhibition of bacterial growth is 67%. Pre-treatment of bacterial cells by Photoditazin significantly enhances the inhibitory effect of the laser light. The bacteriostatic action of the red laser radiation on the methicillin-sensitive S. aureus strain is manifested when the doses of 90 J/cm2 is applied. The degree of inhibition of growth of bacterial colonies of the methicillin-resistant S. aureus strain is also significantly higher after the preliminary action of Photoditazin than with the action of only the laser. 

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