The Effect of Pollutants on the Emergence of Rifampicin Resistance in Bacteria of the Pseudomonas Genus
The anthropogenic pressure on ecosystems may contribute to the spread of antibiotic resistance among bacteria in natural conditions. Due to this, the aim of this work was to study the impact of specific biocides, antibiotics, pesticides and heavy metals on the emergence of rifampicin resistant mutants of Pseudomonas putida. To assess the impact of toxic substances on the emergence of rifampicin-resistant mutants the studied substances and rifampicin in the final concentration of 100 ?g/ml were added into nutrient medium before plating. In the course of the experiment the stimulating effects of clopyralid and glyphosate on the process were shown. The most pronounced effect was observed in the case of glyphosate application in the concentration of 0.67 mg/ml. The obtained results indicate high risk of spreading antibiotic resistance among the microbial communities of soils subjected to treatment with pesticides.
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