Pseudomonas putida

A microbiological study of soil with pesticides burial places in the Saratov region and identified the dominant microbial populations. The number of microorganisms analysis determined that the dominant group in the soil were heterotrophic bacteria and most sensitive – microscopic fungi. Obtained destructors effective microorganisms that break down pesticides prometrin, hexachlorocyclohexane and dihlordifeniltrihlormetilmetan (4,4-DDT). Strains of Pseudomonas putida P2, P. putida P6, P.

The anthropogenic pressure on ecosystems may contribute to the spread of antibiotic resistance among bacteria in natural conditions. Due to this, the aim of this work was to study the impact of specific biocides, antibiotics, pesticides and heavy metals on the emergence of rifampicin resistant mutants of Pseudomonas putida. To assess the impact of toxic substances on the emergence of rifampicin-resistant mutants the studied substances and rifampicin in the final concentration of 100 ?g/ml were added into nutrient medium before plating.

The article presents the development of methodological approaches to increasing destructive potential of Pseudomonas putida SU12 native strain extracted from phenol-contaminated soils. The cyclic selective method on gradual increase of phenol concentration in optoelectronics environment in combination with intermediate stage of accumulative culture is described. The authors proposed a discrete, stepwise increase in the concentration of phenol in elective media depending on degree of its destruction.