Izvestiya of Saratov University.

Chemistry. Biology. Ecology

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ISSN 2541-8971 (Online)

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Changes in the Permeability of the Blood-Brain Barrier in the Development of Alzheimer’s Disease in Mice

Zinchenko Ekaterina M., Saratov State University
Klimova Maria M., Saratov State University
Shirokov Aleksandr A., Institute of Biochemistry and Physiology of Plants and Microorganisms of the Russian Academy of Sciences
Novolokin Nikita A., Saratov State Medical University
Martinov Dmitry V., Saratov State University
Antonova Tatyana S., Saratov State University
Blohina Inna A., Saratov State University
Agranovich Ilana M., Saratov State University
Terskov Andrey V., Saratov State University
Semyachkina-Glushkovskaya Oksana V., Saratov State University

In our study on mice with an injection model of Alzheimer’s disease (AD), beta-amyloid detection in the brain tissue was evaluated using immunohistochemistry and confocal analysis. An accumulation of toxic protein was shown in different areas of the brain, predominantly in the cortex and in the hippocamp (place of beta-amyloid injection). Neurofunctional tests were conducted to confirm the development of AD. It is uncovered that accumulation of ?-amyloid in the brain was associated with development of the sensory, motor, coordinating disjunctions, and also with memory impairment in tested mice. Confocal analysis of the blood-brain barrier (BBB) permeability to fluorescent beta-amyloid revealed a significant leakage of beta-amyloid from the cerebral vessels into the brain tissues in mice with AD. The results indicate the effectiveness of the use of the Alzheimer’s injection model in mice to study the non-genetic mechanisms of the development of a neurodegenerative disease associated with impaired BBB function.

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