Izvestiya of Saratov University.

Chemistry. Biology. Ecology

ISSN 1816-9775 (Print)
ISSN 2541-8971 (Online)
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Language: 
Russian
Heading: 
Biology
Article type: 
Article
UDC: 
577.151
DOI: 
10.18500/1816-9775-2021-21-3-298-303

Obtaining viable Azospirillum brasilense SR80 cells encapsulated in alginate hydrogel

Autors: 
Kovrizhnikov Aleksandr Viktorovich, Saratov State University
Pylaev Timofey E., Institute of Biochemistry and Physiology of Plants and Microorganisms of the Russian Academy of Sciences - Subdivision of the Federal State Budgetary Research Institution Saratov Federal Scientific Centre of the Russian Academy of Sciences (IBPPM RAS)
Zakharevich Andrey Machailovich, Saratov State University
Konnova Svetlana A., Saratov State University
Kupryashina Maria Alexandrovna, Institute of Biochemistry and Physiology of Plants and Microorganisms of the Russian Academy of Sciences - Subdivision of the Federal State Budgetary Research Institution Saratov Federal Scientific Centre of the Russian Academy of Sciences (IBPPM RAS)
Abstract: 

Significant researches aimed at the greening of agro-industrial production are focused on obtaining immobilized bacterial preparations with preserved proliferative function and metabolic activity. Herein, we investigated the possibility of bacteria of the genus Azospirillum to be immobilized in Ca-alginate beads. A. brasilense SR80 cells, encapsulated in an alginate hydrogel, were obtained using the “soft” immobilization method based on physical binding. We demonstrated the retained respiratory activity and growth ability of the bacteria during immobilization, thus confirming the advantageous prospects of alginate templates for azospirilla encapsulation

Key words: 
Azospirillum
alginate
immobilization
encapsulation
viability
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Received: 
05.05.2021
Accepted: 
14.05.2021
Published: 
30.09.2021