lipopolysaccharide

Lipopolysaccharide is the main structural component of the outer membrane of Gram-negative bacteria, which can also be a part of extracellular polymeric substances. Lipopolysaccharides of bacteria that stimulate the growth and development of plants belong to the group of molecules that form a microbe-associated molecular pattern (MAMP). These glycoconjugates of both symbiotic and phytopathogenic bacteria induce the activation of immune responses in plants.

Lipopolysaccharides are compounds of bacterial origin that have biological activity against plants, animals and humans. This work provides information on the preparation and characterization of the properties of modifi ed lipopolysaccharide derivatives of the rhizosphere bacterium Ochrobactrum cytisi IPA7.2. Deacylation has been carried out using alkaline hydrolysis, followed by chromatographic separation of the fractions. O-deacetylation of O-polysaccharide led to a 2-fold increase in the extinction of the products of the phenol-sulfuric acid reaction.

Lipopolysaccharides (LPS) of plant-growth-promoting bacteria of the genus Azospirillum are involved in the initial steps of formation of the association with host plant. This work aimed compositional analysis of the LPS from Azospirillum zeae and Azospirillum formosense. The LPS were extracted from the cells by hot phenolwater solution. Biopolymer composition of the LPS was studied by colorimetric methods. Determination of the fatty acid composition of the lipid A was performed by GLC. O-deacylation of the LPS A.

The greatest diagnostic significance for tularemia is played by antibodies against lipopolysaccharide (LPS). Previously, it has been shown that specific antibodies that appear in the blood serum of laboratory animals infected with virulent strains and people who have recovered from tularemia can bind both to LPS Francisella tularensis and to LPS Francisella novicida, while after the vaccination specific antibodies to LPS F. novicida are absent.