Effect of the O-Antigen Chemical Structure in Different Subspecies of Francisella Tularensis on Immunological Reactions

The greatest diagnostic significance for tularemia is played by antibodies against lipopolysaccharide (LPS). Previously, it has been shown that specific antibodies that appear in the blood serum of laboratory animals infected with virulent strains and people who have recovered from tularemia can bind both to LPS Francisella tularensis and to LPS Francisella novicida, while after the vaccination specific antibodies to LPS F. novicida are absent. The aim of this work was to investigate the effect of the structure of the O-antigen of lipopolysaccharide F.

Peculiarities of the Structure of Azospirillum zeae and Azospirillum formosense Lipopolysaccharides

Lipopolysaccharides (LPS) of plant-growth-promoting bacteria of the genus Azospirillum are involved in the initial steps of formation of the association with host plant. This work aimed compositional analysis of the LPS from Azospirillum zeae and Azospirillum formosense. The LPS were extracted from the cells by hot phenolwater solution. Biopolymer composition of the LPS was studied by colorimetric methods. Determination of the fatty acid composition of the lipid A was performed by GLC. O-deacylation of the LPS A.