For citation:
Golubev D. M., Nesterkina D. D., Tarasyuk A. K., Glinskaya O. S., Pleshakova E. V., Glinskaya E. V., Nechayeva O. V. The substrate spectrum of native hydrocarbon-oxidizing bacteria isolated from soils with diff erent levels of anthropogenic impact. Izvestiya of Saratov University. Chemistry. Biology. Ecology, 2025, vol. 25, iss. 1, pp. 118-123. DOI: 10.18500/1816-9775-2025-25-1-118-123, EDN: YTPZRA
The substrate spectrum of native hydrocarbon-oxidizing bacteria isolated from soils with diff erent levels of anthropogenic impact
The substrate spectrum of hydrocarbon-oxidizing bacteria isolated from the soils of the Saratov region was studied: the soils of Balakovo (chronic anthropogenic pollution) and agricultural soils of Novokrivovka village (single hydrocarbon pollution). It was found that the microorganisms isolated from the urban soils of Balakovo, being under constant anthropogenic infl uence, had a wider substrate spectrum. It was revealed that 4 microorganisms isolated from the urban soils of Balakovo: Acinetobacter lwoffi i R44, Bacillus circulans E75, B. horikoshii P22, Ochrobactrum gallinifaecis I59, and also Paenibacillus polymyxa S31, isolated from the soil of Novokrivovka village, were capable of decomposing the largest amount of hydrocarbon substrates, including crude oil and individual hydrocarbons. 100% of bacterial strains isolated from the soils of Novokrivovka village and 75% of microorganisms isolated from the soils of Balakovo used crude oil as the only carbon source. The number of bacteria capable of using aromatic hydrocarbons for growth was 2–3 times higher among isolates from the soils of Balakovo. Native hydrocarbon-oxidizing bacteria with a wide substrate spectrum can be used to design a biological product aimed at carrying out restoration activities of contaminated soil ecosystems.
- Созина И. Д., Данилов А. С. Микробиологическая ремедиация нефтезагрязненных почв // Записки Горного института. 2023. Т. 260. С. 297–312. http://dx.doi.org/10.31897/PMI.2023.8
- Lv Y., Bao J., Zhu L. A comprehensive review of recent and perspective technologies and challenges for the remediation of oil-contaminated sites // Energy Reports. 2022. Vol. 8. P. 7976–7988. https://doi.org/10.1016/j.egyr.2022.06.034
- Menshakova M. Y., Moskvin K. K., Khodjaliev S. A. Study of the dynamics of oil destruction with the use of bacterial biological preparations in the conditions of the far North // BIO Web of Conferences. 2023. Vol. 63. P. 05011. https://doi.org/10.1051/bioconf/20236305011
- Определитель бактерий Берджи : в 2 т. Т. 2 / под ред. Дж. Хоулт. М. : Мир, 1997. 368 с.
- Руководство к практическим занятиям по микробиологии : практ. пособие / под ред. Н. С. Егорова. 2-е изд. М. : Изд-во Моск. ун-та, 1983. 251 с.
- Das A., Das N., Rajkumari J., Pandey P., Pandey P. Exploring the bioremediation potential of Bacillus spp. for sustainable mitigation of hydrocarbon contaminants // Environmental Sustainability. 2024. P. 1–22. https://doi.org/10.1007/s42398-024-00309-9
- Коршунова Т. Ю., Мухаматдьярова С. Р., Логинов О. Н. Новый штамм бактерии рода Ochrobactrum: свойства и филогенетическое положение // Известия Уфимского научного центра РАН. 2013. № 2. С. 90–94.
- Al-Hadithi H. T., Al-Razzaq E. A., Fadhil G. F. Bioremediation of polycyclic aromatic hydrocarbons by Acinetobacter species isolated from ecological sources // Journal of Environmental Biology. 2017. Vol. 38, № 5. P. 785. http://doi.org/10.22438/jeb/38/5/MRN-422
- Grady E. N., MacDonald J., Liu L., Richman A., Yuan Z.-H. Current knowledge and perspectives of Paenibacillus: A review // Microbial Cell Factories. 2016. Vol. 15. P. 1–18. https://doi.org/10.1186/s12934-016-0603-7