The substrate spectrum of hydrocarbon-oxidizing bacteria isolated from the soils of the Saratov region was studied: the soils of Balakovo (chronic anthropogenic pollution) and agricultural soils of Novokrivovka village (single hydrocarbon pollution). It was found that the microorganisms isolated from the urban soils of Balakovo, being under constant anthropogenic infl uence, had a wider substrate spectrum. It was revealed that 4 microorganisms isolated from the urban soils of Balakovo: Acinetobacter lwoffi i R44, Bacillus circulans E75, B.