Izvestiya of Saratov University.

Chemistry. Biology. Ecology

ISSN 1816-9775 (Print)
ISSN 2541-8971 (Online)

For citation:

Markina N. E., Zakharevich A. M., Markin A. V. Electrochemical SERS study of some endogenous components of human biofl uids. Izvestiya of Saratov University. Chemistry. Biology. Ecology, 2022, vol. 22, iss. 3, pp. 292-301. DOI: 10.18500/1816-9775-2022-22-3-292-301

This is an open access article distributed under the terms of Creative Commons Attribution 4.0 International License (CC-BY 4.0).
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Language: 
Russian
Heading: 
Chemistry
Article type: 
Article
UDC: 
543.424.2
DOI: 
10.18500/1816-9775-2022-22-3-292-301

Electrochemical SERS study of some endogenous components of human biofl uids

Autors: 
Markina Natalia Evgenievna, Saratov State University
Zakharevich Andrey Machailovich, Saratov State University
Markin Aleksey Viktorovich, Saratov State University
Abstract: 

The work describes electrochemical (EC) protocol suitable for preparation of copper electrodes which can be used as substrates in surface-enhanced Raman spectroscopy (SERS). These SERS-active electrodes have been used for electrospectral studies based on the combination of electrochemical and SERS analysis (EC-SRS analysis). Several endogenous bodyfl uid components (urea, creatinine, uric acid, bilirubin) have been selected for the study because they can signifi cantly aff ect the SERS-based determination of other analytes in bodyfl uids (for example, drugs). The infl uence of the SERS-active electrode polarization (applied potential) and the pH level of the analyte solutions on the SERS signal and current value have been investigated. The polarization values corresponded to the maximum SERS signal are observed at negative values for all analytes (below −0.2 V vs. copper pseudo-reference electrode). The maximal SERS signal has been observed for most of the analytes in a neutral medium (at the optimum polarization value of the SERS-active electrode), and the weakest signal has been in an alkaline medium. The diminishing of EC-SERS signal at high pH values is explained by deprotonation of analyte molecules that deteriorates analyte adsorption onto the negatively polarized SERS-active electrodes. Analysis of the current-voltage curves has been used to estimate the possible infl uence of EC changes of the studied molecules on their EC-SERS signal. The results obtained in this work will be useful for the development of EC-SERS systems suitable for the determination of various endo- and exogenous compounds in human biofl uids.

Key words: 
surface-enhanced Raman spectroscopy
urea
creatinine
uric acid
bilirubin
SERS-active electrodes
copper SERS substrates
electrochemical cell
electrospectral system
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Received: 
29.03.2022
Accepted: 
09.04.2022
Published: 
30.09.2022