Izvestiya of Saratov University.

Chemistry. Biology. Ecology

ISSN 1816-9775 (Print)
ISSN 2541-8971 (Online)


For citation:

Galitskaya A. A., Galitskaya A. A., Nikitina V. E., Babitskaya V. G., Shcherba V. V., Puchkova T. A. Characterization of Glycoproteinaceous Metabolites from the Xylotrophic Basidiomycete Lentinus edodes under Submerged and Surface Cultivation. Izvestiya of Saratov University. Chemistry. Biology. Ecology, 2010, vol. 10, iss. 1, pp. 29-35. DOI: 10.18500/1816-9775-2010-10-1-29-35

This is an open access article distributed under the terms of Creative Commons Attribution 4.0 International License (CC-BY 4.0).
Full text:
(downloads: 32)
Language: 
Russian
Heading: 
Article type: 
Article
UDC: 
579.22.577.112.083

Characterization of Glycoproteinaceous Metabolites from the Xylotrophic Basidiomycete Lentinus edodes under Submerged and Surface Cultivation

Autors: 
Galitskaya Anna A., Saratov State University
Galitskaya A. A., Institute of Biochemistry and Physiology of Plants and Microorganisms of the Russian Academy of Sciences
Nikitina V E, Institute of Biochemistry and Physiology of Plants and Microorganisms of the Russian Academy of Sciences
Abstract: 

The exo- and endoglycopolymers of the fungus L. edodes were found to be glycoproteins  that, by  their monomeric  composition, were hete- roglycans  with glucose as  the predominant monomer. The structure of the exo- and endoglycoproteins formed by the fungus was found to depend to some extent on the cultivation method used. The carbohy­ drate moieties  of the exo- and endoglycoproteins  were represented by  branched glycans  containing a- and 0-glycosidic  links. The main chain was  represented by  glycans  with the Ci—>Сз glycosidic  links, and the sidechains contained glycans with the Ci—>Сд and Ci—>Ce glycosidic links.

Reference: 

1. 

By Т. Благоприятные лечебные эффекты экзотических съедобных грибов // Бюл. МАГ. 1996. С. 12-17.

2. 

Bender S.Lonergan G.T.Backhaus J.Cross R.F., Dumi-trach-Anghel C.N. The antibiotic activity of the edible and medicinal mushroom Lenlinus edodes (Berk.) Sing. // Intern. J. Medicinal Mushrooms. 2001. Vol.3. P.118.

3. 

Белова Н.В. Перспективы использования биологически активных соединений высших базидиомицетов в России // Микол. и фитопатол. 2004. Т.38, вып.2. С. 1-7.  EDN: MPRRIF

4. 

Tang Y.J., ZhongJ.J. Fed-batch fermentation of Ganoderma lucidum for hyperproduction of polysaccharide and ganoderic acid // Enzyme and Microbial Technology. 2002. Vol. 31. P.20-28.  EDN: AXWGBH

5. 

Грушеико MM. Совместное использование фенол-сернокислотного и толуидинового способов определения Сахаров как метод изучения углеводного состава лигноуг-леводного комплекса // Лигноуглеводные комплексы древесины. Рига, 1978. С.32-35.

6. 

Babitskaya V.G.Scherba V.V.Mitropolskaya N.Y. Exopo-lysaccharides of some medicinal mushrooms: production and composition // Intern. J. Medicinal Mushrooms. 2000. Vol.2. P.51-54.

7. 

Lowry O.M. Protein measurement with the Folin phenol reagent /7 J, Biol. Chem. 1951. №193. P.265-275.

8. 

Bradford M.M. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding // Anal. Biochem. 1976. Vol.72, №l-2.P.248-254.

9. 

Бробст K.M. Методы исследования углеводов / Под ред. А.Я. Хорлина. М, 1975. 445 с.

10. 

Gutierrez A.Prieto A.Martinez A. T. Structural characterization of extracellular polysaccharides produced by fungi from the genus Pleurotus If Carbohydrate Research, 1996. Vol.281. P. 143-154.

11. 

Mizuno T. The extraction and development of antitumour-active polysaccharides from medicinal mushrooms // Intern. J. Medicinal Mushrooms. 1999. Vol.1, №1. P.9-29.

Received: 
02.11.2009
Accepted: 
10.01.2010
Published: 
25.02.2010
Short text (in English):
(downloads: 18)