цветометрия

The extraction of the tungsten blue complex formed by the reaction of phenol with Folin-Ciocalteu reagent (FCR) in the presence of a nonionic surfactant Triton X-100 in alkaline medium using the methodology of micellar extraction based on «clouding point» has been studied for the fi rst time. Aqueous solutions of sodium carbonate and sodium sulfate were used to create pH and as a desalting agent.

The brackish microalgae Dunaliella salina, being an extremophilic halophyte, is a promising object for biotechnological production. The aim of this work is to develop a methodology for non-destructive control of the development of a microalgae culture under conditions of balanced growth during periodic cultivation on plates. Before the start of the experiment, the microalgae culture was synchronized. The quantitative content of chlorophylls a and b, as well as carotenoids, was determined spectrophotometrically in alcohol extracts.

В настоящей работе исследована возможность разделения бинарных и многокомпонентных смесей – аминокислот методом зонального электрофореза на целлюлозной поддерживающей среде с последующей визуализацией выделенных зон и цветометрической обработкой полученных сигналов.

For the efficient preconcentration of azo compounds – products of the interaction of 4-nitrophenyldiazonium with thymol, a system 4-nitroaniline (4-NA) – NO2 - – Triton X-100 – NaOH – ethanol has been proposed. The optimal conditions for the formation of micellarsaturated phases of the system under study have been established: 3. 10-4 M 4-NA – 3. 10-4 M NO2 - – 5% Triton X-100 – 2.8 M NaOH – 10 vol. % С2Н5ОН. A spectrophotometric study of the above system has been carried out.

Test means based on immobilized Fehling’s reagent for the determination of cephalexin have been obtained. Methods for visual and colorimetric assessment of antibiotic concentration using a smartphone camera have been developed. The optimal conditions for the indicator reaction have been selected with varying heating time and temperature. For a visual semi-quantitative assessment of the content of cephalexin, a color scale has been obtained.