Izvestiya of Saratov University.

Chemistry. Biology. Ecology

ISSN 1816-9775 (Print)
ISSN 2541-8971 (Online)


Full text:
(downloads: 316)
Language: 
Russian
Heading: 
Article type: 
Article
UDC: 
543.05 : 547.3

Determination of thymol with micellar extraction preconcentration

Autors: 
Tsygulyova Elmira I., Saratov State University
Doronin Sergey Yu., Saratov State University
Abstract: 

For the efficient preconcentration of azo compounds – products of the interaction of 4-nitrophenyldiazonium with thymol, a system 4-nitroaniline (4-NA) – NO2 - – Triton X-100 – NaOH – ethanol has been proposed. The optimal conditions for the formation of micellarsaturated phases of the system under study have been established: 3. 10-4 M 4-NA – 3. 10-4 M NO2 - – 5% Triton X-100 – 2.8 M NaOH – 10 vol. % С2Н5ОН. A spectrophotometric study of the above system has been carried out. A linear dependence was built in the coordinates A (at ?max = 552 nm) vs с(thymol), which is described by an equation of the form A = f(c), A = 26291c + 0.02; R2 = 0.997. The range of the determined contents of thymol is (2·10-6 – 4·10-5) mol/l. A technique for the colorimetric determination of thymol in aqueous media (color channel G) has been developed. The intensity of the channel G chromaticity (I G ) is linearly dependent on pc(thymol) in accordance with the equation I G= 54.2pc – 267, R 2 = 0; the lower limit of the determined contents of thymol is 1. 10-6 mol/l, which is two times less than in the variant of its spectrophotometric determination. The profiles of petal diagrams in the color coordinates of the RGB CMYK model have been constructed; the dependences of their area (S) and perimeter (P) on the thymol concentration have been obtained (P: y = 278x – 10.13; R 2 = 0.97; S: y = 20182x – 87649, R 2 = 0.99).

Reference: 
  1. Ziyatdinova G. K., Romashkina S. A., Ziganshina E. R., Budnikov H. C. Voltammetric Determinations of Thymol on an Electrode Modifi ed by Coimmobilized Carboxylated Multiwalled Carbon Nanotubes and Surfactants. J. Anal. Chem., 2018, vol. 73, no. 1, рр. 52–59 (in Russian). https://doi.org/10.7868/S0044450218010073
  2. Suleymanov T., Balayeva E. Development of HPLC methods for the quantitative determination of thymol in syrup «KALINOL PLUS». International Scientifi c Review, 2016, vol. 14, no. 4, рр. 215–217 (in Russian).
  3. Stepanyuk S. N., Nikitina N. V., Nikitina A. S., Borovskiy B. V. Development of a method for the quantitative determination of thymol in the essential oil of monarda fumous and dental fi lms. Young scientists and pharmacy of the XXI century: Collection of scientifi c papers the fourth scientifi c and practical conference with international participation, (Moscow, December 16, 2016). Moscow, Federal State Budgetary Scientifi c Institution “All-Russian Research Institute of Medicinal and Aromatic Plants”, 2016, рр. 320–322 (in Russian).
  4. Mika J., Barek J., Zima J., Dejmkova H. New fl owthrough coulometric detector with renewable working electrode material for fl ow injection analysis and HPLC. Electrochim. Acta, 2015, vol. 154, рр. 397–403. https:// doi.org/10.1016_j.electacta.2014.12.091
  5. Cantalapiedra A., Gismera M. J., Sevilla M. T., Procopio J. R. Sensitive and selective determination of phenolic compounds from aromatic plants using an electrochemical detection coupled with HPLC method. Phytochem. Anal., 2014, vol. 25, no. 3, рр. 247–254. https://doi.org/10.1002/pca.2500
  6. Zima J., Cienciala M., Barek J., Moreira J.C. Determination of thymol using HPLC-ED with glassy carbon paste electrode. Chemia Analityczna, 2007, vol. 52, no. 6, рр. 1049–1057.
  7. Khorokhordina E. A., Tran Hai Dang. Extraction methods of phenolic ecotoxicants and their determination in materials and environmental objects. Scientifi c Bulletin of the Voronezh State University of Architecture and Civil Engineering, 2014, vol. 8, no. 1, рр. 93–105 (in Russian).
  8. Zhestovskaya E. S., Doronin S. Yu. «Cloud point» мicellar ecxtraction as a mode for phenols concentration. J. Butlerov Communications, 2016, vol. 45, no. 2, pp. 66–81 (in Russian). https://doi.org/jbc-01/16-45-2-66
  9. Chernova R. K., Doronin S. Yu. Opredelenie organicheskikh analitov v rastvorakh PAV: ionnye i mitselyarnye effekty [Determination of Organic Analytes in Surfactant Solutions: Ionic and Micellar Effects]. Saratov, Izd-vo Sarat. un-ta, 2017. 200 р. (in Russian).
  10. Doronin S. Yu., Zhestovskaya E. S., Tsyguleva E. I. Micellar Extraction Preconcentration and Colorimetric Determination of Some Phenols. J. Anal. Chem., 2020, vol. 75, no. 6, pp. 502–509 (in Russian). https://doi.org/10.31857/S0044450220060079
Received: 
13.04.2021
Accepted: 
14.05.2021
Published: 
30.09.2021