Izvestiya of Saratov University.

Chemistry. Biology. Ecology

ISSN 1816-9775 (Print)
ISSN 2541-8971 (Online)

Full text:
(downloads: 92)
Article type: 

Competitive Binding of K+ in the Presence of Na+ with Bovine Serum Albumin and Hemoglobin

Rebrov Viktor Georgievich, Saratov State University
Verkhov Dmitry Gennadievich, Saratov State University
Sidorenko Svetlana Vadimovna, Lomonosov Moscow State University
Usanov Andrey Dmitrievich, Saratov State University
Skripal Anatoly Vladimirovich, Saratov State University
Usanov Dmitry Aleksandrovich, Saratov State University

Selective accumulation of monovalent cations K+, Na+, Rb+, Cs+ with albumin and hemoglobin is shown. Preferential sorption of potassium in the presence of sodium with test proteins is determined. It is shown that with increasing concentrations of sodium in the composition of aqueous solutions of the proteins, the amount of potassium bound by proteins increases. The results of investigation concerning sorption of potassium ions in the presence of sodium ions at various concentrations of theirs chloride salts by bovine serum albumin and hemoglobin coincide with similar results observed in studies on living muscle cells.


1. Phillips L. A., Hotham-Iglewski B., Franklin R. M. Polyribosomes of Escherichia coli: I. Effects of monovalent cations on the distribution of polysomes, ribosomes and ribosomal subunits // J. Mol. Biol. 1969. Vol. 40, № 2. P. 279-288.

2. Reisner A. H., Bucholtz C. Studies on polyribosomes of Paramecium : I. Effect of Monovalent Ions // Exp. Cell. Res. 1972. Vol. 73, № 2. P. 441-455.

3. Kroeger H. Zellphysiologische Mechanismen bei der Regulation von Genaktivitaten in den Riesenchromosomen von Chironomus thummi // Chromosoma. 1964. Vol. 15, № 1. P. 36-70.

4. Lezzi M. Differential gene activation in isolated chromosomes // Intern. Rev. Cytol. 1970. Vol. 29, № 1. P. 127-168.

5. Lezzi M., Gilbert L. J. Differential effects of K+ and Na+ on specifi c bands of isolated polytene chromosomes of Chironomus tentans // J. Cell Sci. 1970. Vol. 6. P. 615-627.

6. Витухновская Л. А., Исмаилов А. Д. Влияние ионов Na+, K+ на люминесцентную активность интактных клеток Vibrio harveyi при различных pH // Микробиология. 2001. Т. 70, № 4. С. 525-530.

7. Бояндин А. Н., Попова Л. Ю. Зависимое от минеральных солей ингибирование свечения люминесцентного микроорганизма Escherichia coli Z905 // Биофизика. 2001. Т. 26, № 2. С. 251-255.

8. Дерябин Д. Г., Алешина Е. С., Каримов И. Ф. Влияние катионов K+, Na+, Mg2+, Ca2+ на активность бактериальных биолюминесцентных систем in vitro и in vivo // Вестн. ОГУ. Прил. Биоэлементология. 2006. № 12. С. 77-82.

9. Fujiwara-Nagata E., Kogure K., Kita-Tsukamoto K., Wada M., Eguchi M. Characteristics of Na+-dependent respiratory chain in Vibrio anguillarum, a fi sh pathogen, in comparison with other marine Vibrios // FEMS Microbiol. Ecology. 2003. Vol. 44, № 2. P. 225-230.

10. Szent-Gyorgyi A. Studies on muscle // J. Acta Physiol. Scandinavica. 1945. № 9. Suppl. 25. P. 115.

11. Lewis M. S., Saroff H. A. The Binding of Ions to the Muscle Proteins. Measurements on the Binding of Potassium and Sodium Ions to Myosin A, Myosin B and Actin // J. Amer. Chem. Soc. 1957. №79. P. 2112-2117.

12. Szenkuti L., Giese W. Studies on the Binding Capacity of the Skeletal Muscle Constituents For Cesium-134 // J. Health Physics. 1974. № 26. P. 343-347.

13. Beatley E. H., Klotz I. M. Interaction of sodium and potassium ions with hemoglobin and with hemerythrin // J. Biol. Bull. 1951. № 101. P. 215.

14. Carr C. W. Studies on the binding of small ions in protein solutions with the use of membrane electrodes. VI. The binding of sodium and potassium ions in solutions of various proteins // J. Arch. Biochem. Biophys. 1956. № 62. P. 476-484.

15. Edelmann L. Potassium adsorption sites in frog muscle visualized by cesium and thallium under the transmission electron microscope // J. Physiol. Chem. Phys. 1977. № 9. P. 313-317.

16. Trombitas C., Tigyi-Sebes A. X-ray microanalytical studies on native myofi brils and mitochondria isolated by microdissection from honey-bee fl ight muscle // Acta Biochimica et Biophysica; Academiae Scientiarum Hungaricae. 1979. №14. P. 271-277.

17. Edelmann L., Fresenius Z. Selective Accumulation of Li+, Na+, K+, Rb+, and Cs+ at Protein Sites of Freeze-Dried Embedded Muscle Detected by LAMMA // J. Anal. Chem. 1981. № 308. P. 218-220.

18. Zglinicki T. von. Monovalent Ions are Spatially Bound within the Sarcomere // J. Gen. Physiol. Biophys. 1988. № 7. P. 495-504.

19. Ling G. N. A revolution in the Physiology of the Living Cell. Malabar : Fl. Krieger Publ. Co., 1992. 404 р.

20. Ling G. N., Ochsenfeld M. M. Studies on ion accumulation in muscle cells // J. Gen. Physiol. 1966. Vol. 49, № 4. P. 819-843.

21. Eisenman G. Glass Electrodes for Hydrogen and Other Cations: Principles and Practices. N.Y. : Marcel Dekker, 1967. 344 p.

22. Ling G. N. A new model for the living cell : a summary of the theory and recent experimental evidence in its support // J. Intern. Rev. Cytology. 1969. № 26. P. 1-61.

23. Гордон Дж. Органическая химия растворов электролитов. М. : Мир, 1979. 712 с.

24. Мархол М. Ионообменники в аналитической химии. Свойства и применение в неорганической химии : в 2 ч. Ч. 1. М. : Мир, 1985. 264 с.

25. Ling G. N. A Physical Theory of the Living State : The Association-Induction Hypothesis. Waltham, Massachusetts: Blaisdell Publ. Co., 1962. 680 p.

26. Балданов М. М., Балданова Д. М., Жигжитова С. Б., Танганов Б. Б. К проблеме радиусов гидратированных ионов // ДАН ВШ России. 2006. № 2. С. 32-34.

27. Широбоков И. Б., Вельма Н. А. Моделирование строения гидратных оболочек ионов щелочных металлов и галогенид-ионов статистическими методами // Вестн. Удмурт. ун-та. Сер. Физика. Химия. 2008. Вып. 2. С. 37-57.

28. Debye P., Pauling L. The inter-ionic attraction theory of ionized solutes. IV. The infl uence of variation of dielectric constant on the limiting law for small concentrations // J. Amer. Chem. Soc. 1925. № 47. P. 2129- 2134.

29. Ling G. N. Quantitative relationships between the concentration of proteins and the concentration of K+ and Na+ in red cell ghosts // Phosphorus Metabolism. Vol. II / eds. W. D. McElroy, B. Glass. Baltimore : The Johns Hopkins Univ. Press., 1952. P. 748-795.

30. Ling G. N. All-or-none adsorption by living cells and model protein-water systems: discussion of the problem of «permease-induction» and determination of secondary and tertiary structures of proteins // Fed. Proc. 1966. Vol. 25, № 3. P. 958-970.