Izvestiya of Saratov University.

Chemistry. Biology. Ecology

ISSN 1816-9775 (Print)
ISSN 2541-8971 (Online)
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Language: 
Russian
Heading: 
Biology
Article type: 
Article
DOI: 
10.18500/1816-9775-2018-18-1-52-59

Rhizosphere Microorganisms’ Collection of IBPPM RAS: Revision of Azospirillum Strains Based on 16S rRNA Gene Sequence Analysis

Autors: 
Turkovskaya Olga V., Institute of Biochemistry and Physiology of Plants and Microorganisms of the Russian Academy of Sciences - Subdivision of the Federal State Budgetary Research Institution Saratov Federal Scientific Centre of the Russian Academy of Sciences (IBPPM RAS)
Golubev Sergey N., Institute of Biochemistry and Physiology of Plants and Microorganisms of the Russian Academy of Sciences - Subdivision of the Federal State Budgetary Research Institution Saratov Federal Scientific Centre of the Russian Academy of Sciences (IBPPM RAS)
Dubrovskaya Ekaterina V., Institute of Biochemistry and Physiology of Plants and Microorganisms of the Russian Academy of Sciences - Subdivision of the Federal State Budgetary Research Institution Saratov Federal Scientific Centre of the Russian Academy of Sciences (IBPPM RAS)
Abstract: 

The IBPPM Collection maintains unique pool of bacterial strains related to Azospirillum genus, notable for its biotechnological potential. Progressively developing systematics of this genus requires checking and clarifying the taxonomic position for long-term stored isolates in the collection. Consequently, the species membership was confirmed or defined in the tested Azospirillum strains using a comparative analysis of their full-length 16S rRNA genes combined with morphological, cultural, physiological and biochemical properties, as well as DNA-DNA hybridization data. Members of two proposed new Azospirillum species were found.

Key words: 
Azospirillum
16S rRNA gene
DNA-DNA hybridization.
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