Izvestiya of Saratov University.

Chemistry. Biology. Ecology

ISSN 1816-9775 (Print)
ISSN 2541-8971 (Online)

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Exopolysaccharide-Producing Halophilic and Halotolerant Microorganisms Isolated from the Saline Lakes Qarun (Egypt) and Elton (Russia)

Ibrahim Ibrahim M., Saratov State University
Konnova Svetlana A., Saratov State University
Sigida Elena N., Institute of Biochemistry and Physiology of Plants and Microorganisms of the Russian Academy of Sciences
Fedonenko Yulia P., Institute of Biochemistry and Physiology of Plants and Microorganisms of the Russian Academy of Sciences
Safronova Vera I., All-Russia Research Institute for Agricultural Microbiology

Microorganisms inhabiting saline environments can produce compounds unique in structure and properties. This ability recently prompted researchers to study the biotechnological potential of halophiles. This work aimed at screening the exopolysaccharideproducing halophilic and halotolerant microorganisms of two saline lakes, one in in Egypt and the other in Russia. For strain isolation and characterization, we used microbiological and physico-chemical methods, as well as light and transmission electron microscopy. Taxonomy studies of the isolates was studied on the basis of analyses of cultural and morphological characteristics, biochemical properties and 16S rDNA sequencing data. Of 104 isolates adapted to saline environments we selected 49 exopolysaccharide-producing strains. The content of polysaccharides in the culture liquids of the isolated microorganisms reached 13.7 mg/ml-1. Sixteen isolates were identified to species.


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