Izvestiya of Saratov University.

Chemistry. Biology. Ecology

ISSN 1816-9775 (Print)
ISSN 2541-8971 (Online)
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Language: 
Russian
Heading: 
Biology
Article type: 
Article
UDC: 
612.82:616-073
DOI: 
10.18500/1816-9775-2018-18-4-423-428

Optimization of Photodynamic Effects on Permeability of Cerebral Vessels

Autors: 
Semyachkina-Glushkovskaya Oksana V., Saratov State University
Shirokov Aleksandr A., Institute of Biochemistry and Physiology of Plants and Microorganisms of the Russian Academy of Sciences - Subdivision of the Federal State Budgetary Research Institution Saratov Federal Scientific Centre of the Russian Academy of Sciences (IBPPM RAS)
Novolokin Nikita A., Saratov State Medical University
Terskov Andrey V., Saratov State University
Bodrova Anastasiya A., Saratov State University
Shushunova Natalia A., Saratov State University
Khorovodov Aleksandr P., Saratov State University
Ulanova Maria V., Saratov State University
Agranovich Ilana M., Saratov State University
Iskra Tatyana D., Saratov State University
Mamedova Aysel T., Saratov State University
Abstract: 

Photodynamic treatment (PDT) causes a significant increase in the permeability of the blood-brain barrier (BBB) in healthy mice. Using different doses of laser radiation (635 nm, 10–40 J/cm2 ) and photosensitizer (5-aminolevulinic acid – 5-ALA, i.v.), we found the optimal PDT for the reversible opening of the BBB, exhibiting brain tissue recovery 3 days after PDT. Further increases in the laser radiation or 5-ALA doses have no amplifying effect on the BBB permeability, but are associated with severe damage of brain tissues. These results can be an informative platform for further studies of new strategies in brain drug delivery and for better understanding of mechanisms underlying cerebrovascular effects of PDT-related resection of brain tumor.

Key words: 
photodynamic treatment
blood-brain barrier
lasers
endothelial permeability
Reference: 

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