Chlorophyllin Inhibits Chemiluminescence That Accompanies a Quasi-Hypoxygenase Reaction Catalyzed by the Cytochrome c-Cardiolipin Complex
Lipid peroxidation is a key factor in programmed cell death of various etiologies. This process also occurs when ionizing radiation acts on biological systems. It is the essence of the theory of lipid radiotoxins, which is part of the structural and metabolic theory of the biological action of ionizing radiation. Non-enzymatic lipid peroxidation eventually leads to death by the mechanism of ferroptosis, while enzymatic lipid peroxidation, catalyzed by the cytochrome c-cardiolipin complex, triggers apoptosis along the mitochondrial pathway. According to the results of research conducted over the past 10 years, it has been established that drugs based on chlorophyll are effective as radioprotectors. The only mechanism of their action may be the inhibition of radical reactions involving lipids. Therefore, drugs based on chlorophyll can be used as the antioxidants in the prevention and treatment of various pathologies caused by free radical lipid oxidation. In the present study, using the method of activated coumarin-334 (quinolysidine[5,6,7- gh]3-acetylcoumarin) chemiluminescence, we have established the suppression of the reaction of radical lipid oxidation caused by the quasilipoxygenase activity of the cytochrome c-cardiolipin complex. This conclusion was arrived at on the basis of reliable suppression of chemiluminescence by sodium chlorophyllin at concentrations of 1.56 ?m and higher. The obtained result shows the relevance of further multi-faceted research of the possibility of effective use of various chlorophyll derivatives in the treatment and prevention of pathological conditions caused by oxidative stress.
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