Izvestiya of Saratov University.

Chemistry. Biology. Ecology

ISSN 1816-9775 (Print)
ISSN 2541-8971 (Online)


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Boyko A. S., Smolkina O. N., Konnova S. A., Fedonenko Y. P., Ignatov V. V. Characterization of the Lipopolysaccharide of the Diazotrophic Rhizobacterium Azospirillum Brasilense Sr55. Izvestiya of Saratov University. Chemistry. Biology. Ecology, 2007, vol. 7, iss. 1, pp. 60-63.

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Characterization of the Lipopolysaccharide of the Diazotrophic Rhizobacterium Azospirillum Brasilense Sr55

Autors: 
Boyko Alevtina Sergeevna, Institute of Biochemistry and Physiology of Plants and Microorganisms of the Russian Academy of Sciences - Subdivision of the Federal State Budgetary Research Institution Saratov Federal Scientific Centre of the Russian Academy of Sciences (IBPPM RAS)
Smolkina Olga Nikolaevna, Institute of Biochemistry and Physiology of Plants and Microorganisms of the Russian Academy of Sciences - Subdivision of the Federal State Budgetary Research Institution Saratov Federal Scientific Centre of the Russian Academy of Sciences (IBPPM RAS)
Konnova Svetlana A., Saratov State University
Fedonenko Yulia P., Institute of Biochemistry and Physiology of Plants and Microorganisms of the Russian Academy of Sciences - Subdivision of the Federal State Budgetary Research Institution Saratov Federal Scientific Centre of the Russian Academy of Sciences (IBPPM RAS)
Ignatov V. V., Institute of Biochemistry and Physiology of Plants and Microorganisms of the Russian Academy of Sciences - Subdivision of the Federal State Budgetary Research Institution Saratov Federal Scientific Centre of the Russian Academy of Sciences (IBPPM RAS)
Abstract: 

This work was undertaken to investigate the lipopolysaccharide from gram-negative, plant-growth-promoting diazotrophic rhizobacterium Azospirillum brasilense SR55 isolated from the roots of wheat in Saratov oblast. The lipopolysaccharide was extracted from the outer membrane with phenol, and its chemical makeup was studied. The major components of the hydrophobic portion included 3-hydroxytetradecanoic, 3-hydroxyhexadecanoic, hexadecanoic, and octadecanoic acids; those of the carbohydrate portion included thamnose, galactose, 3-O-methithamnose, and glucuronic acid. The repeated unit of the O-specific polysaccharide of A. brasilense SR55 was found to be a branched octasaccharide with a glucuronic acid residue in its sidechain.

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Reference: 
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Received: 
30.08.2006
Accepted: 
20.11.2006
Published: 
25.02.2007
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