For citation:
Boyko A. S., Smolkina O. N., Konnova S. A., Fedonenko Y. P., Ignatov V. V. Characterization of the Lipopolysaccharide of the Diazotrophic Rhizobacterium Azospirillum Brasilense Sr55. Izvestiya of Saratov University. Chemistry. Biology. Ecology, 2007, vol. 7, iss. 1, pp. 60-63.
Characterization of the Lipopolysaccharide of the Diazotrophic Rhizobacterium Azospirillum Brasilense Sr55
This work was undertaken to investigate the lipopolysaccharide from gram-negative, plant-growth-promoting diazotrophic rhizobacterium Azospirillum brasilense SR55 isolated from the roots of wheat in Saratov oblast. The lipopolysaccharide was extracted from the outer membrane with phenol, and its chemical makeup was studied. The major components of the hydrophobic portion included 3-hydroxytetradecanoic, 3-hydroxyhexadecanoic, hexadecanoic, and octadecanoic acids; those of the carbohydrate portion included thamnose, galactose, 3-O-methithamnose, and glucuronic acid. The repeated unit of the O-specific polysaccharide of A. brasilense SR55 was found to be a branched octasaccharide with a glucuronic acid residue in its sidechain.
- Konnova S.A., Makarov O.E., Skvortsov I.M., Ignatov V.V. Isolation, fractionation and some properties of polysaccharides produced in a bound from by Azospiriltum brasilense and their possible involvement in Azospirilhtm-wheat root interaction // FEMS Microbiol. Lett. 1994. V. 118. P.93-99. EDN: PSDDPF
- Yegorenkova I.V., Konnova S.A., Sachuck V.N., Ignatov V. V. Azospirilhtm brasilense colonization of wheat roots and role of lectin-carbohydrate interactions in bacterial adsorption and root-hair deformation //Plant Soil. 200J. V.23I. P.275-282. EDN: LGRELF
- Федоненко Ю.П., Егоренкова И.В., Коннова С.А., Игнатов В.В. Участие липополисахаридов азоспирилл во взаимодействии с поверхностью корней пшеницы // Микробиология. 2001. Т.70, №3. С.384-390. EDN: MPGUZL
- Newman M.A., Dow J.M., Daniels M.J. Bacterial lipopolysaccharides and plant-pathogen interactions // Europ. J. Plant Pathology. 2001. V.107. P.95-102. EDN: AMLOVB
- Позднякова Л.И., Каневская С.В., Леванова Г.В. и др. Таксономическое изучение азоспирилл, выделенных из злаков Саратовской области // Микробиология. 1988. Т.57, №2. С.275-278. EDN: WLFZEH
- Вестфаль О., Янн К. Бактериальные липополисахариды // Методы химии углеводов. М.: Мир, 1967. С.325-332.
- Hakomori S.-I. A rapid permetylation of glycolypids and polysaccharides catalyzed by methylsulfinyl carbanion in demethylsulfoxide//J. Biochem. 1964. V.55. P.205-208. EDN: IRBODB
- Hitchcock P.J., Brown T.M. Morphological heterogeneity among Salmonella lypopolysaccharide chemotypes in silver-stain polyacrylamide gels // J. Bacteriol. 1983. V. 154. P.269-277.
- Коннова С.А., Федоненко Ю.П., Игнатов В.В. Структура и функции гликополимеров поверхности азоспирилл // Молекулярные основы взаимоотношений ассоциативных микроорганизмов с растениями. М.: Наука, 2005. С.42-63. EDN: KGPJCY
- Choma A., Russa R., Mayer H., Lorkiewicz Z. Chemical analysis of Azospirillum lypopolysaccharides // Arch. Microbiol. 1987. V.146. P.341-345.
- Федоненко Ю.П., Здоровенко Э.Л., Коннова С.А. и др. Сравнительная характеристика липополисахаридов и О-специфических полисахаридов Azospirillum brasilense Sp245 и его Омегон-Km-мутантов КМ018 и КМ252 // Микробиология. 2004. Т.73, №3. С. 180-187. EDN: LIIEET