direct organogenesis

Regeneration of plants in vitro through direct organogenesis allows the avoidance of somaclonal variation. The aim of our study was the induction of direct organogenesis in the culture of mature embryo in maize lines created at Saratov State University: ATTM (bm, wx, y), ATTM (bm, y), ATTM (bm, g, V-type CMS), AT-3 (4n). These lines are characterized by genetic predisposition to parthenogenesis. The mature embryos isolated from sterile grains were used as a primary explant.

The article presents the results of the search for the optimal hormonal composition of the nutrient medium for the induction of direct organogenesis in the culture of mature embryos of the maize homozygous line ATTM (bm, wx, y). The mature embryos isolated from kernels were used as the primary explant. The 11 media variants were tested for induction of direct organogenesis: MS without hormones (control) and with phytohormones in different concentrations and combinations.