Izvestiya of Saratov University.

Chemistry. Biology. Ecology

ISSN 1816-9775 (Print)
ISSN 2541-8971 (Online)


For citation:

Simonenko A. V., Savelyev G. K., El-Khih A. N., Khanadeev V. A., Tuchina E. S. Gold nanostars as a photoagent under the antimicrobial action of infrared (808 nm) laser radiation. Izvestiya of Saratov University. Chemistry. Biology. Ecology, 2023, vol. 23, iss. 1, pp. 86-93. DOI: 10.18500/1816-9775-2023-23-1-86-93, EDN: GVNYVU

This is an open access article distributed under the terms of Creative Commons Attribution 4.0 International License (CC-BY 4.0).
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Russian
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Article
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577.344.3.579.61.666.1.056
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GVNYVU

Gold nanostars as a photoagent under the antimicrobial action of infrared (808 nm) laser radiation

Autors: 
Simonenko Andrey V., Saratov State University
Savelyev Grigoriy K., Saratov State University
El-Khih Ayya Nidal, Saratov State University
Khanadeev Vitaly A., Institute of Biochemistry and Physiology of Plants and Microorganisms of the Russian Academy of Sciences
Tuchina Elena S., Saratov State University
Abstract: 

Gold nanostars with an average core diameter of 122,2 nm and a spike length of 114,6 nm were synthesized and characterized at a concentration of 5,36×1010 pcs/ml with an absorption maximum of 840 nm. Gold nanostars were coated with thiolated polyethylene glycol, its amount was about 8×104 molecules per 1 particle and about 4,4×1015 molecules/ml in the colloid. The zeta potential of gold nanostars coated with PEG-SH was −2.3 mV. The combined eff ect of gold nanostars and low-intensity infrared (808 nm) laser radiation on the bacteria Staphylococcus aureus 209 P and Escherichia coli 113-13 has been studied. Incubation of suspensions of microorganisms in the presence of nanoparticles without access to light did not lead to a signifi cant reduction in the number of bacteria. Irradiation for 30 min of bacterial suspensions containing nanoparticles caused the death of 39% of the S. aureus population and 80% of the E. coli population. During the thermometry of the studied suspensions, it was found that the temperature increase is dose-dependent. The increase in temperature of the control samples that did not contain photothermal agents did not exceed 1 °C in both cases throughout the entire time of the experiment. For suspensions of bacteria (equally S. aureus and E. coli) incubated with gold nanostars during irradiation, an increase in temperature indicators, on average, by 4 °C was revealed. The more pronounced antibacterial activity of the combination of gold nanostars and infrared (808 nm) laser radiation against E. coli can also be explained by the greater sensitivity of gram-negative microorganisms to photothermal exposure.

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Received: 
03.11.2022
Accepted: 
07.12.2022
Published: 
31.03.2023
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