Izvestiya of Saratov University.

Chemistry. Biology. Ecology

ISSN 1816-9775 (Print)
ISSN 2541-8971 (Online)

For citation:

Bazarnov E. V., Богомолов А. Ю. Flow-through photometric cell for online determination of the biomass content of microalgae Nannochloropsis sp. in the cultural environment. Izvestiya of Saratov University. Chemistry. Biology. Ecology, 2025, vol. 25, iss. 3, pp. 254-263. DOI: 10.18500/1816-9775-2025-25-3-254-263, EDN: FAMVVG

This is an open access article distributed under the terms of Creative Commons Attribution 4.0 International License (CC-BY 4.0).
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Language: 
Russian
Heading: 
Chemistry
Article type: 
Article
UDC: 
543.07
DOI: 
10.18500/1816-9775-2025-25-3-254-263
EDN: 
FAMVVG

Flow-through photometric cell for online determination of the biomass content of microalgae Nannochloropsis sp. in the cultural environment

Autors: 
Bazarnov Evgenii Vyatcheslavovitch, Samara State Technical University
Богомолов Андрей Юрьевич, Samara State Technical University
Abstract: 

One of the promising directions of carbon-neutral energy development is the development of technologies for biofuel production from microalgae. Eff ective control of biotechnological processes in the fi eld of cultivation of such microalgae requires express and eff ective methods of process control. In this study, a fl ow-through photometric cell has been designed and fabricated for online determination of the biomass content of the microalgae Nannochloropsis sp. in the culture medium. The microalgae biomass has been cultured in a custom-made photobioreactor. Using the fabricated cell, a series of photometric measurements of solutions of the grown biomass in the culture medium have been made. A linear dependence of absorbance on biomass content has been obtained from the results of measurements. The possibility of online determination of the biomass content of microalgae Nannochloropsis sp. in the culture medium using the developed fl ow-through photometric cell based on a laser with a working wavelength of 650 nm has been shown.

Key words: 
biomass content
online monitoring
fl ow photometric cell
cultivation of Nannochloropsis sp.
Arduino
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Received: 
27.01.2025
Accepted: 
04.04.2025
Published: 
30.09.2025
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