For citation:
Dashtoian J. V., Kalinina A. V., Korobko V. V. Effect of salinity on the morphogenesis of seedlings of introgressive lines of spring bread wheat with genetic material Aegilops columnaris. Izvestiya of Saratov University. Chemistry. Biology. Ecology, 2023, vol. 23, iss. 3, pp. 308-317. DOI: 10.18500/1816-9775-2023-23-3-308-317, EDN: FVXSFT
Effect of salinity on the morphogenesis of seedlings of introgressive lines of spring bread wheat with genetic material Aegilops columnaris
The introduction of the genetic material of wild-growing species into the genome of cultivated plants can signifi cantly expand the diversity of breeding material. For the eff ective use of introgressive plants, it is necessary to study their economically valuable traits and adaptive capabilities. The objective of the work is to study the eff ect of two types of salinity on the morphogenesis of seedlings of introgressive lines of spring bread wheat containing the genetic material of Aegilops columnaris Zhuk. The objects of study are seedlings of spring bread wheat of the Dobrynya variety and seedlings of introgressive lines of spring bread wheat, created in the laboratory of genetics and cytology of the Federal Center of Agriculture Research of the South-East Region on the basis of the Dobrynya variety and Aegilops columnaris Zhuk. Cultivation of plants was carried out on solutions of sodium chloride and sulfate; the concentrations of the solutions corresponded to an osmotic pressure of 7 atm. The eff ect of salinity on growth was assessed by the following indicators: root index value, root-to-shoot ratio of seedlings, length of the fi rst leaf, and number of roots of seven-day-old seedlings. Among the studied introgressive lines of spring bread wheat, the highest resistance to the action of sodium chloride is characteristic of seedlings of lines L1946/1 (6A(6U); T4BS/T4BL-1D; 3AL with terminal C-band), L1882/2 (monosomic addition 2/4/ 7X), L2021/4 (5D(5X)6D(6X); terminal transl. 3DL) and L2021/2 (5D(5X)6A(6X)). Seedlings of lines L1882/2 (monosomic addition 2/4/7X) and L2028/3 (5D(5X)) showed resistance to sulfate salinity. Seedlings of line L2028/3 (5D(5X)) are slightly inferior to seedlings of the Dobrynya variety in the length of the fi rst leaf under conditions of chloride salinization, and line L2021/4 is inferior in terms of root supply under conditions of sulfate salinity (5D(5X)6D(6X); terminal transl. 3DL). The results obtained allow us to conclude that the indicated changes in the genome have a positive or no negative eff ect on the salt tolerance of common wheat. The pronounced negative eff ect of chloride and sulfate salinity on the studied morphometric parameters was found in plants of the line L1721/2 (6A(6Udel); T4BS/T4BL-1D), which suggests that such a change in the genome negatively aff ects the salt tolerance of bread wheat.
- Дружин А. Е., Сибикеев С. Н., Гультяева Е. И., Баранова О. А., Андреева Л. В., Голубева Т. Д., Калинцева Т. В. Изучение хозяйственно ценных и адаптивных признаков у линий яровой мягкой пшеницы, созданных с участием синтетических форм пшеницы // Аграрный вестник Юго-Востока. 2019. № 3. С. 7–10.
- Коробко В. В., Волков Д. П. Устойчивость некоторых сортов зернового сорго к разнокачественному засолению // Известия Саратовского университета. Новая серия. Серия: Химия. Биология. Экология. 2013. Т. 13, вып. 2. С. 107–111.
- Badaeva E. D., Ruban A. S., Shishkina A. A., Sibikeev S. N., Druzhin A. E., Surzhikov S. A., Dragovich A. Yu. Genetic classifi cation of Aegilops columnaris Zhuk. (2n = 4x = 28, UcUcXcXc) chromosomes based on FISH analysis and substitution patterns in common wheat × Ae. columnaris introgressive lines // Genome. 2018. Vol. 61, № 2. P. 131–143. https://doi.org/10.1139/gen-2017-0186
- Ledo А., Burslem D. F. R. P., Paul K. I., Battaglia M., England J. R., Pinkard E., Roxburgh S., Ewel J. J., Barton C., Brooksbank K., Carter J., Eid T. H., Fitzgerald A., Jonson J., Mencuccini M., Montagu K. D., Montero G., Ruizpeinado R., Mugasha W. A., Ryan C. M., Sochacki S., Specht A., Wildy D., Wirth C., Zerihun A., Chave J. Tree size and climatic water defi cit control root to shoot ratio in individual trees globally // New Phytologist. 2018. Vol. 217, № 1. P. 8–11. https://doi.org/10.1111/ nph.14863
- Шапошников А. И., Моргунов А. И., Акин Б., Макарова Н. М., Белимов А. А., Тихонович И. А. Сравнительные характеристики корневых систем и корневой экссудации у синтетического, примитивного и современного сортов пшеницы // Сельскохозяйственная биология. 2016. Т. 51, № 1. С. 68–78. https://doi.org/10.15389/agrobiology.2016.1.68rus
- Pal N., Saini D. K., Kumar S. Meta-QTLs, ortho-MQTLs and candidate genes for the traits contributing to salinity stress tolerance in common wheat (Triticum aestivum L.) // Physiology and Molecular Biology of Plants. 2021. Vol. 27, № 12. Р. 2767–2786. https://doi.org/10.1007/s12298-021-01112-0
- Юдина Р. С., Леонова И. Н., Салина Е. А., Хлесткина Е. К. Изменение солеустойчивости мягкой пшеницы в результате интрогрессии генетического материала Aegilops speltoides и Triticum timopheevii // Вавиловский журнал генетики и селекции. 2015. Т. 19, № 2. С. 171–175. https://doi.org/10.18699/VJ15.021
- Huang S., Spielmeyer W., Lagudah E. S., James R. A., Platten J. D., Dennis E. S., Munns R. A sodium transporter (HKT7) is a candidate for Nax1, a gene for salt tolerance in durum wheat // Plant Physiology. 2006. Vol. 142, № 4. P. 1718–1727. https://doi.org/1104/ pp.106.088864