Izvestiya of Saratov University.

Chemistry. Biology. Ecology

ISSN 1816-9775 (Print)
ISSN 2541-8971 (Online)

For citation:

Kanevsky M. V., Menukhov V. O., Kosheleva I. S., Kostritsky A. Y., Kanevskaya I. V., Konnova S. A. Changes in the physicochemical and cultural properties of the bacteria Azospirillum baldaniorum Sp245 under the infl uence of some synthetic coumarins. Izvestiya of Saratov University. Chemistry. Biology. Ecology, 2022, vol. 22, iss. 2, pp. 215-225. DOI: 10.18500/1816-9775-2022-22-2-215-225

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Language: 
Russian
Heading: 
Biology
Article type: 
Article
UDC: 
579.222+579.262
DOI: 
10.18500/1816-9775-2022-22-2-215-225

Changes in the physicochemical and cultural properties of the bacteria Azospirillum baldaniorum Sp245 under the infl uence of some synthetic coumarins

Autors: 
Kanevsky Matvey V., Saratov State University
Menukhov Vladisla O., Federal State Budgetary Institution of Science, Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences
Kosheleva Irina S. , Saratov Medical Research Center for Hygiene of the “Federal Scientifi c Center for Medical and Preventive Health Risk Management Technologies”
Kostritsky A. Yu., Saratov State University
Kanevskaya Irina V., Saratov State University
Konnova Svetlana Anatolyevna, Saratov State University
Abstract: 

Due to the small number of studies on the role of coumarins in associative symbiotic relationships, some aspects of the infl uence of synthetic coumarins on the physicochemical and cultural properties of Azospirillum baldaniorum Sp245 were studied for the fi rst time. To reveal the role of hydroxylation in position 7 of the fused aromatic ring – 1-(2-oxo-2H-chromen-3-yl)butan-1,3-dione, comparative studies of the eff ect of the original and hydroxylated coumarins on the culture of a model strain of azospirilla were carried out. The survival of bacteria after the addition of coumarins was studied by counting CFU on an agar medium. The biofi lm formation activity of the culture was assessed using crystal violet. The change in the surface of bacteria under the action of coumarins was studied by the electrical polarizability of bacterial cells on an ELUS electrooptical analyzer (EloSystemGbR, Germany). The yield and monosaccharide composition of extracellular glycopolymers were studied using gas-liquid chromatography.For the fi rst time, an increase in the yield of EPS of bacteria during growth in the presence of 1-(7-hydroxy-2-oxo-2Hchromen-3-yl)butan-1,3-dione by 1.2 and 1.7 times for concentrations of 50 and 100 μM respectively was observed. It has been established for the fi rst time that the hydroxylated substance has a higher antibacterial activity compared to the unsubstituted one. A decrease in the number of viable cells in planktonic culture and inhibition of biofi lm growth were revealed. It has been shown by electro-optical analysis that the presence of coumarins in the cultivation medium in all concentrations studied leads to a change in the electrical polarizability of A. baldaniorum Sp245 cells. The use of electrooptical analysis of cell suspensions using monospecifi c antibodies obtained against lipopolysaccharides of this strain made it possible to reveal the absence of changes in carbohydrate antigenic determinants on the surface of bacterial cells. This is consistent with the data of the analysis of the composition of extracellular polysaccharides by GLC, during which no diff erences were found in the qualitative composition and ratio of monosaccharides. An increase in the yield of bacterial EPS during growth in the presence of 1-(7-hydroxy-2-oxo-2H-chromen-3-yl) butan-1,3-dione by 1.2 and 1.7 times for concentrations of 50 and 100 μM was shown. The results obtained allow us to consider the changes that have occurred as features of the adaptation of bacteria to the associative conditions of existence.

Key words: 
Azospirillum baldaniorum
coumarins
extracellular polysaccharides
electrooptical analysis
biofi lms
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Received: 
31.01.2022
Accepted: 
07.02.2022
Published: 
30.06.2022
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