Izvestiya of Saratov University.

Chemistry. Biology. Ecology

ISSN 1816-9775 (Print)
ISSN 2541-8971 (Online)

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Zhylko V. V., Nekhan N. В. Application of cationic dye pyronin G (Y) for quantitative extraction-photometric determination of higher carboxylic acids in fi sh. Izvestiya of Saratov University. Chemistry. Biology. Ecology, 2022, vol. 22, iss. 3, pp. 267-274. DOI: 10.18500/1816-9775-2022-22-3-267-274

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Application of cationic dye pyronin G (Y) for quantitative extraction-photometric determination of higher carboxylic acids in fi sh


The relevance of this study is due to the need to develop methods for the extraction and quantitative determination of higher carboxylic acids in fi sh. In this work, we propose a simple, inexpensive, and highly sensitive extraction-photometric technique for the selective determination of the total content of fatty acids. This technique is selective and is based on the quantitative extraction into the organic phase of ionic associates of the cationic dye pyronin G (Y) with highly hydrophobic carboxylic acids and seems promising for the quantitative determination of the fatty carboxylic acids in fi sh. Considerable attention is paid to the sample preparation, which is the most important stage of analysis, since the work is carried out with a natural sample that has a complex composition. The technique has been tested on real objects, also using this technique, a directly proportional dependence of the concentration of higher carboxylic acids in fi sh on the storage time has been obtained. It has been established that the heptane/iso-propanol system is the most eff ective for the extraction of higher carboxylic acids. The limit of detection of higher carboxylic acids by the extraction-photometric method using the cationic dye pyronin G(Y) in fi sh is 4,4 10-7 M. The results obtained allow us to state that the developed method can be used to determine the freshness of fi sh.

  1. ГОСТ 7631-2008. Рыба, нерыбные объекты и продукция из них. Взамен ГОСТ 7631-85; 2009 – 01 – 01. Минск : Межгос. Совет по стандартизации, метрологии и сертификации; М. : Изд-во стандартов, 2010. 16 с.
  2. Боева Н. П. Технология жиров из водных биологических ресурсов. М. : Изд-во ВНИРО, 2016.107 с.
  3. Alberta N. FTIR determination of free fatty acids in fi sh oils intended for biodiesel production // Process Biochemistry. 2009. Vol. 44. P. 401–405. https://doi. org/10.1016/j.procbio.2008.12.004
  4. Seppanen-Laakso Т. Analysis offatty acids by gas chromatography, and its relevance to research on health and nutrition // Analytica Chimica Acta. 2002. Vol. 465. P. 39–62. https://doi.org/10.1016/S0003- 2670(02)00397-5
  5. Abalos M. Application of gas chromatography coupled to chemical ionisation mass spectrometry following headspace solid-phase microextraction for the determination of free volatile fatty acids in aqueous samples // J. of Chromatography A. 2000. Vol. 891. P. 287–294. https:// doi.org/10.1016/S0021-9673(00)00655-5
  6. Nikolova-Damyanova B. High-performance liquid chromatography of fatty-acid derivatives in the combined silver ion and reversed-phase modes. // J. of Chromatography A. 1993. Vol. 653, № l. P. 15–23. https://doi. org/10.1016/0021-9673(93)80387-N
  7. Brando T. Analysis of aminofl uorescein-fatty acid derivatives by capillary electrophoresis with laser-induced fl uorescence detection at the attomole level: application to mycobacterial fatty acids // J. of Chromatography A. 2002. Vol. 973. P. 203–210. https://doi.org/10.1016/ S0021-9673(02)01216-5
  8. Windarsih A., Lestari L., Erwanto Y., Putri A. R., Fadzillah N. A., Rahmawati N., Rohman A. Application of Raman Spectroscopy and Chemometrics for Quality Controls of Fats and Oils: A Review // Food Reviews International. 2021. P. 1–20. https://doi.org/10.1080/87 559129.2021.2014860
  9. Aziz N. A. Quantitative Determination of Fatty Acids in Marine Fish and Shellfi sh from Warm Water of Straits of Malacca for Nutraceutical Purposes // BioMed Research International. 2013. P. 1–12. https://doi. org/10.1155/2013/284329
  10. Khoddami A. Quality and fatty acid profi le ofthe oil extracted from fi sh waste (head, intestine and liver) (Euthynnus affi nis) // African Journal of Biotechnology. 2012. Vol. 11. P. 1683–1689. https://doi.org/10.5897/ AJB10.1699
  11. Otero P., Carpena M., Fraga-Corral M., GarciaOliveira P., Soria-Lopez A., Barba F. J., Xiao J., SimalGandara J., Prieto M. A. Aquaculture and agricultureby products as sustainable sources of omega-3 fatty acids in the food industry // eFood. 2021. Vol. 2, № 5. P. 209–233. https://doi.org/10.53365/efood.k/144603
  12. Crexi V. T. Winterization of fi sh oil with solvent // Food Science and Technology. 2009. Vol. 29, № 1. P. 207–213. https://doi.org/10.1590/S0101-20612009000100032
  13. Homayooni B. Concentrations of omegs-3 fatty acids from rainbow sardine fi sh oil by various methods // International Food Research Journal. 2015. Vol 5, № 2. P. 743–748.
  14. Ali S. K., Shahidi F., Sedaghat N. Evaluation of the effect of carboxy methyl cellulose edible coating containing Astragalus honey (Astragalus gossypinus) on the shelflife of pistachio kernel // Food Control. 2022. 109094. https://doi.org/10.1016/j.foodcont.2022.109094
  15. Iverson S. Comparison of the Bligh and Dyer and Folch Methods for Total Lipid Determination in a Broad Range of Marine Tissue // Lipids. 2001. Vol. 36. P. 1283–1287. https://doi.org/10.1007/s11745-001-0843-0
  16. Hara A. Lipid Extraction of Tissues with a LowToxicity Solvent // Analytical Biochemistry. 1978. Vol. 90. P. 420–426. https://doi.org/10.1016/0003- 2697(78)90046-5
  17. Gunnlaugsdottir H. Three extraction methods for determination of lipids in fi sh meal: Evaluation of a hexane/ isopropanol method as an alternative to chloroform-based methods // Journal ofthe Science of Food and Agriculture. 1993. Vol. 61. P. 235–240. https://doi.org/10.1002/ jsfa.2740610216
  18. Жилко В. В. Экстракция высших карбоновых кислот с катионным красителем Пиронин Ж в сильнощелочной среде // Аналитика РБ-2018 : сб. ст. 6-й республ. конф. по аналит. химии. Минск : Колорград, 2018. С. 70.
  19. Жилко В. В. Подбор катионных красителей и условий экстракции для фотометрического определения высших карбоновых кислот // Актуальные задачи химии: исследования и перспективы : сб. материалов конф. Житомир : Изд-во ЖГУ им. И. Франкo,