It is known that bacterial alginates are widely used in biomedicine as carriers in the immobilization of cells, enzymes and drugs. Their functional properties depend on their monomeric composition and molecular weight and vary depending on the source and the cultivation conditions. It was established that molasses (waste of sugar beet production) can be used as an effective and cheap source of nutrition for the growth of the bacterium Azotobacter vinelandii strain D-05 and the production of alginate.