Most microorganisms in natural conditions exist in the form of biofilms. Due to this fact, the question of research of various substances influence on their formation process is relevant. This article presents data on the influence of various groups of pollutants, such as antibiotics (ampicillin, oxytetracycline), antiseptics (dioxidine, chlorhexidine), herbicides (glyphosate, clopyralid) and salts of heavy metals (CuSO4, HgCl2) in various concentrations on the intensity of biofilm formation by the strain Vibrio aquamarinus VKPM B-11245.