The Influence of Growth Conditions of Bacteria Azospirillum lipoferum Sp59b on the Biological Activity of Their Glycopolymers
It is well known that lipopolysaccharides (LPSs) from outer membranes and capsular polysaccharides of Gram-negative bacteria activate in- nate immune system of humans and mammals and can produce septic shock clinical signs. A beneficial effect of LPSs consists in moderate stimulation of production of endogenous mediators by immune system cells, thus increasing the resistance of the organism to infections. In this respect the development of non-toxic derivatives of bacterial glycopolymers with improved immunostimulary properties becomes urgent. Therefore, the aim of our research was to evaluate the influence of the growth conditions of bacteria Azospirillum lipoferum Sp59b on the biological activity of their glycans. Using the experimental models of mouse macrophages and human whole blood cells (in vitro), we made a comparative study on the immunostimulatory properties of eight glycopolymers of A. lipoferumSp59b grown under different cultivation conditions. For the first time, it has been shown that biopolymer and fatty acid compositions of these glycanes depend on the source of nitrogen and carbon and on the growth phase. It was elucidated that alteration of growth conditions led to significant rearrangements in the lipid A structures of the LPSs and dramatically changed their ability to stimulate mouse macrophage enzymes and synthesis of the main proinflomatory cytokines in human whole blood cells.
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