Laser-induced Vasomotor Responses on Chorioallantoic Membrane
The strength and the specific featu res of the vasomotor responses provide important information about the healthy state of the blood vessel, since it s contraction or dilation is caused by the activation or relaxation of vascular smooth muscle cells of the vascular wall and appears to be a universal response to the a number of physiological regulatory pathways, including humoral and neurogenic ones. While the methods for testing the isolated vessel fragment ex vivo are well developed and widely used in research, a similar problem with respect to a functioning microcirculatory network is much more complicated and largely unresolved. In this paper, we investigate the possibilities of a new method of to test a fragment of an arterial vessel within the network. Namely, we study the vasomotor reactions of a single arterial vessel of a chick embryo chorioallantoic membrane in situ induced by laser irradiation. Our results show that the reaction type (dilatation or constriction) depends essentially on the wavelength of the laser radiation. In a number of cases, a biphasic response is detected, when initial dilatation upon repeated exposures is replaced by constriction. The developed technique is proposed to be used as a nondestructive and non-contact experimental technique for the studies of microcirculatory network functions.
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