Investigation of Interaction Between Silicon-Based Nanoparticles and HeLa Cells
This work is about the investigation of the interaction of porous silicon submicron particles with the HeLa cervical cancer cell line. Substrates of the porous silicon were obtained with the two-staged chemical method based on synthesis of silver nanoparticles from silver nitrate AgNO3 with the subsequent etching with fluoric acid HF in the presence of hydrogen peroxide H2O2 and mechanical separation of microparticles by shattering. The thickness of the obtained porous silicon layer measured by scanning electrom microscopy (SEM), was about 2 mkm. The size of separate particles in the layer, measured by the semicontact atomic force microscopy method was about 200 nm. Testing of metabolic cell activity for cytotoxity and biocompatibility of the obtained particles was done with Alasar Blue dye. Metabolic activity was measured by fluorescence of the sample with the excitation wavelength 560 nm and detection wavelength 590 nm. Nanoparticles do not show noticeable cytotoxicity with the HeLa cell line despite using silver nanoparticles and fluoric acid as intermediate reagents for their making. There is some increase in metabolic activity in the all samples containing submicron particles compared to the control sample.
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