Izvestiya of Saratov University.

Chemistry. Biology. Ecology

ISSN 1816-9775 (Print)
ISSN 2541-8971 (Online)

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Investigation of Interaction Between Silicon-Based Nanoparticles and HeLa Cells

Terin Denis V., Saratov State University
Bratashov Daniil N., Saratov State University
Revzina Elena M., Saratov State University
Rokakh Gleb E., Saratov State University

This work is about the investigation of the interaction of porous silicon submicron particles with the HeLa cervical cancer cell line. Substrates of the porous silicon were obtained with the two-staged chemical method based on synthesis of silver nanoparticles from silver nitrate AgNO3 with the subsequent etching with fluoric acid HF in the presence of hydrogen peroxide H2O2 and mechanical separation of microparticles by shattering. The thickness of the obtained porous silicon layer measured by scanning electrom microscopy (SEM), was about 2 mkm. The size of separate particles in the layer, measured by the semicontact atomic force microscopy method was about 200 nm. Testing of metabolic cell activity for cytotoxity and biocompatibility of the obtained particles was done with Alasar Blue dye. Metabolic activity was measured by fluorescence of the sample with the excitation wavelength 560 nm and detection wavelength 590 nm. Nanoparticles do not show noticeable cytotoxicity with the HeLa cell line despite using silver nanoparticles and fluoric acid as intermediate reagents for their making. There is some increase in metabolic activity in the all samples containing submicron particles compared to the control sample.


1. Durnev A. D., Solomina A. S., Daugel-Dauge N. O., Zhanataev A. K., Shreder E. D., Nemova E. P., Shreder O. V., Veligura V. A., Osminkina L. A., Timoshenko V. Y., Seredenin S. B. Evaluation of genotoxicity and reproductive toxicity of silicon nanocrystals // Bull. Exp. Biol. Med. 2010. Vol. 149, iss. 4. P. 445–454.

2. Zhi Bo, Mishra S., Hudson-Smith N. V., Kortshagen U. R., Haynes Ch. L. Toxicity Evaluation of Boron- and Phosphorus-Doped Silicon Nanocrystals toward Shewanella oneidensis MR-1 // ACS Appl. Nano Mater. 2018. Vol. 1, iss. 9. P. 4884–4893.

3. Fucikova A., Valenta J., Pelant I., Hubalek Kalbacova M., Broz A., Rezek B., Kromka A., Bakaeva Z. Silicon nanocrystals and nanodiamonds in live cells : photoluminescence characteristics, cytotoxicity and interaction with cell cytoskeleton // RSC Adv. 2014. № 4. P. 10334–10342.

4. Najar A., Slimane A. B., Hedhili M. N., Anjum D., Sougrat R. Effect of hydrofl uoric acid concentration on the evolution of photoluminescence characteristics in porous silicon nanowires prepared by Ag – assisted electroless etching method // J. of Appl. Phys. 2012. № 112. P. 1–6.

5. Lin L., Sun G. X. Synthesis and Photoluminescence Properties of Porous Silicon Nanowire Arrays // Nanoscale Res. Lett. 2010. № 5. P. 1822–1828.

6. Iatsunskyi I., Smyntyna V., Pavlenko N., Sviridova O. Peculiarities of Photoluminescence in Porous Silicon Prepared by Metal-Assisted Chemical Etching // ISRN Optics. 2012. Vol. 2012. P. 1–6.

7. Huang J., Jing Z., Yin W., Hui F. Metal-assisted chemical etching of silicon: a review // Adv. Mater. 2011. Vol. 44, iss. 23. P. 285–308.

8. Necas D., Klapetek P. Gwyddion : an open-source software for SPM data analysis // Cent. Eur. J. Phys. 2012. Vol. 10, iss. 1. P. 181–188.

9. Haidary S. M., Corcoles E. P., Ali N. K. Nanoporous Silicon as Drug Delivery Systems for Cancer Therapies // J. of Nanomaterials. 2012. Vol. 2012. ID 830503. P. 1–15.