Izvestiya of Saratov University.

Chemistry. Biology. Ecology

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ISSN 2541-8971 (Online)

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Study of the Morphological Features of the Cells of the Bacteria Yersinia pestis, Grown at Different Temperatures by Atomic Force Microscopy

Utkin Denis V., Saratov State University
Bulgakova Elena G., Russian Research Anti-Plague Institute "Microbe"
Erokhin Pavel S., Russian Research Anti-Plague Institute "Microbe"
Kuznetsov Oleg Svyatoslavovich, Russian Research Anti-Plague Institute "Microbe"
Kuklev Vasily E., Russian Research Anti-Plague Institute "Microbe"
Osina Natalia A., Russian Research Anti-Plague Institute "Microbe"

Atomic force microscopy in the study of bacterial cells allows the determination of such morphological parameters as: length, width, cell thickness, its perimeter, cross-sectional area, volume and root mean square roughness of the cell surface, which depends on the degree of laying of peptidoglycan and density of lipopolysaccharide. The aim of this work was to study the changes in the morphological parameters of the cell and the cell surface of bacteria when the temperature conditions of the culture medium change on the Y. pestis model. Along with the traditional morphological parameters the coefficients characterizing the functional state of cells – plasticity, rigidity of the cell wall were determined. The measurements were carried out using standard methods of semi-contact atomic force microscopy and software analysis of AFM images. On the example of Y. pestis it is shown that an increase in the temperature of bacteria cultivation from 28 °C to 37 °C leads to an increase in the volume of the cell, the surface roughness of the cell, and the rigidity of the cell wall. Lowering the cultivation temperature in vitro from 28 °C to 4 °C leads to loss of the rigidity of the cell wall, smoothing of the surface and increasing plasticity. The obtained results extend the information about the mechanisms of adaptation of the plague microbe in different temperature conditions.


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