Chromatographical Analysis of Meldonium with Preliminary Concentration by Solid-Phase Extraction on Magnetite Nanoparticles
A comprehensive method for meldonium analysis, based on a combination of magnetic solid-phase concentration of the analyte on magnetite nanoparticles modified with cetylammonium bromide (CTAB) cations and its subsequent analysis by thin-layer chromatography (TLC) were developed in the process of study. The influence of a number of factors on meldonium sorption was studied, namely: pH, sorbent mass, sorption and mixing times; optimal extraction conditions were determined. It was found that quantitative sorption proceeded in 20 min with a meldonium extraction degree of 88%. Ethanol was found to be an eluent to desorb the analyte by 95%. The calibration dependences plotted with and without concentration of meldonium were compared. It was established that the use of concentration on two mg of CTAB-modified nanomagnetite reduces the lower limit of the analyzed contents of meldonium in 25 ml of solution by 5 times.
- Gorbunova A. A., Kireev S. Yu., Rashevskaya I. V. Meldonium: relationship of structure, structure and properties. Vestnik of Penza State University, 2017, no. 2 (17), pp. 92-99 (in Russian).
- Vertkin A. L., Khavasova N. O., Pshenichnikova V. V., Alekseev M. A., Abdullaeva A. U. Meldonium: effective action points. Cardiovascular Therapy and Prevention, 2013, vol. 12, no. 2, pp. 94-97 (in Russian).
- Samorodskaya I. V. Meldonium: review of research results. Russian Medical Journal, 2013, vol. 21, no. 6, pp. 1818-1822 (in Russian).
- Saprykin L. V., Serdan A. A., Saprykina L. V. Direct analysis of betaines in biological fl uids by HPLC. Sorption and Chromatography Processes, 2006, vol. 6, no. 1, pp. 114-122 (in Russian).
- Azaryan A. A., Temerdashev A. Z., Dmitrieva E. V. Determination of Meldonium in human urine by HPLC with tandem mass spectrometric detection. Journal of Analytical Chemistry, 2017, vol. 72, no. 10, pp. 1057-1060 (in Russian). DOI: https://doi.org/10.7868/S0044450217100048
- Lv Y.-F., Hu X., Bi K.-S. Determination of mildronate in human plasma and urine by liquid chromatography-tandem mass spectrometry. J. Chromatogr. B, 2007, vol. 852, pp. 35-39. DOI: https://doi.org/10.1016/j.jchromb.2006.12.031
- Cai L.-J., Zhang J., Peng W.-X., Zhu R.-H., Yang J., Cheng G., Wang X.-M. Determination of Mildronate in Human Plasma and Urine by UPLC-Positive Ion Electrospray Tandem Mass Spectrometry. Chromatogr., 2011, vol. 73, pp. 659-665. DOI: https://doi.org/10.1007/s10337-010-1839-8
- Sorokoumov P. N., Savelieva E. I., Karakashev G. V., Kopeikin V. A., Radilov A. S. Determination of meldonium, gamma-butyrobetaine and carnitine in blood plasma by high-performance liquid chromatography with massselective detection. Drug Development and Registration, 2016, no. 1 (14), pp. 176-183 (in Russian).
- Azaryan A. A., Temerdashev A. Z., Kiseleva N. V. Sposob opredeleniya mel’doniya v moche cheloveka [Method for meldonium determination in human urine]. Patent RF № 217.015.F80В, 29.12.2017 (in Russian).
- Ye Peng, Jing Yang, Zhirui Wang, Jingwen Wang, Yu Liu, Zhuo-Jing Luo, Aidong Wen. Determination of mildronate by LC-MS/MS and its application to a pharmacokinetic study in healthy Chinese volunteers. J. Chromatogr. B. Analytical technologies in the biomedical and life sciences, 2010, vol. 878, pp. 551-556. DOI: 10.1016/j. jchromb.2009.12.030
- Wang J., Hu X.-J., Ni K.-Y. Determination of netilmicin sulfate and its related substances by HPLC-ELSD and HPLC-MSn. Journal of Chinese Pharmaceutical Sciences, 2006, vol. 41, pp. 1268–1271.
- Vaz F. M., Melegh B., Bene J., Cuebas D., Gage D. A., Bootsma A., Vreken P., Gennip A. H. van, Bieber L. L., Wanders R. J. A. Analysis of carnitine biosynthesis metabolites in urine by HPLC-electrospray tandem mass spectrometry. Clin. Chem., 2002, no. 48, pp. 826-34. DOI: https://doi.org/10.1093/clinchem/48.6.826
- Mezentseva O. L., Slepchenko G. B. Ispol’zovaniye modifitsirovannykh uglerodsoderzhashchikh elektrodov dlya opredeleniya mel’doniya v biologicheskikh ob”yektakh [Use of modifi ed carbon-containing electrodes for the determination of Meldonium in biological objects]. Analitika Sibiri i Dal’nego Vostoka: materialy X Vseros. nauch. konf. s mezhdunar. uchastiyem [Analytics of Siberia and the Far East: materials of the X all-Russian scientifi c conference with international participation]. September 12-17, Barnaul, 2016. Barnaul, Izd-vo AltGU, 2016, pp. 80-81 (in Russian).
- Mezentseva O. L., Kryukovsky V. P. Opredeleniye i raspoznavaniye mel’doniya i L-karnitina s ispol’zovaniyem grafi tovogo elektroda, modifi tsirovannogo arendiazoniyem [Determination and recognition of Meldonium and Lcarnitine using a graphite electrode modifi ed with arendiazonium]. Khimiya i khimicheskaya tekhnologiya v XXI veke: materialy XX Vseros. nauch.-prakt. konf. im. prof. L. P. Kuleva studentov i molodykh uchenykh s mezhdunar. uchastiyem [Chemistry and chemical technology in the XXI century: materials of the XX all-Russian scientifi c and practical conference named after Professor L. P. Kulev of students and young scientists with international participation]. Tomsk, TPU, 2019, pp. 313 (in Russian).
- Mezentseva O. L., Slepchenko G. B., Cybikova S. B. The possibility of voltammetric determination of meldonium on carbon-containing electrodes. Theoretical and experimental chemistry: Abstracts of the VIth international scientifi c conf. Karaganda, Publ. House of KSU, 2017, pp. 39.
- Gavrilin M. V., Mudretsova Yu. V., Senchenko S. V., Rozhnova S. A. Development of quantifi cation method for determination of choline alphoscerate and meldonium by capillary electrophoresis. Problems of Biological, Medical and Pharmaceutical Chemistry, 2012, vol. 10, no. 4, pp. 12-17 (in Russian).
- Andreu V., Blasco C., Pico Y. Analytical strategies to determine quinolone residues in food and the environment. Trends Anal. Chem., 2007, vol. 26, no. 6, pp. 534-556. DOI: http://dx.doi.org/10.1016/j.trac.2007.01.010
- Bailac S., Ballesteros O., Jimenez-Lozano E., Barron D., Sanz-Nebot V., Navalov A., Vilchez J. L., Barbosa J. Determination of quinolones in chicken tissues by liquid chromatography with ultraviolet absorbance detection. J. Chromatogr. A, 2004, vol. 1029, pp. 145-151. DOI: https://doi.org/10.1016/j.chroma.2003.11.110
- Czyrski A. Analytical Methods for determining third and fourth generation fl uoroquinolones: A review. Chromatographia, 2017, vol. 80, no. 2, pp. 181-200. DOI: https://doi.org/10.1007/s10335
- Watabe S., Yokohama Y., Nakazawa K., Shinozaki K., Hiraoka R., Takeshita K., Suzuki Y. Simultaneous measurement of pazufl oxacin, ciprofl oxacin, and levofl oxacin in human serum by high-performance liquid chromatography with fl uorescence detection. J. Chromatogr. B., 2010, vol. 878, pp.1555-1561. DOI: https://doi.org/10.1016/j.jchromb.2010.04
- Fedotov P. S., Malofeeva G. I., Savonina E. Y., Spivakov B. Y. Solid-Phase Extraction of Organic Substances: Unconventional Methods and Approaches. Journal of Analytical Chemistry, 2019, vol. 74, no. 3, pp. 205-212 (in Russian). DOI: https://doi.org/10.1134/S0044450219030046
- Lucena R., Simonet B. M., Cardenas S., Valcarcel M. Potential of nanoparticles in sample preparation. J. Chromatogr. A, 2011, vol. 1218, no. 4, pp. 620-637. DOI: https://doi.org/10.1016/j.chroma.2010.11.042
- Aguilar-Arteaga K., Rodriguez J. A., Barrado E. Magnetic solids in analytical chemistry: A review. Anal. Chim. Acta, 2010, vol. 674, no. 2, pp. 157-165. DOI: https://doi.org/10.1016/j. aca.2010.06.043
- Wierucka M., Biziuk M. Application of magnetic nanoparticles for magnetic solid-phase extraction in pre-paring biological, environmental and food samples. Trends Anal. Chem., 2014, vol. 59, pp. 50-58. DOI: https://doi.org/10.1016/j.trac.2014.04.007
- Egunova O. R., Konstantinova T. A., Shtykov S. N. Magnetic nanoparticles in separation and preconcentration. Izv. Saratov Univ. (N. S.), Ser. Chemistry. Biology. Ecology, 2014, vol. 14, iss. 4, pp. 27-34 (in Russian).
- Xie L., Jiang R., Zhu F., Liu H., Ouyang G. Application of functionalized magnetic nanoparticles in sample preparation. Anal. Bioanal. Chem., 2014, vol. 406, no. 2, pp. 377-399. DOI: https://doi.org/10.1007/s00216-013-7302-6
- Tolmacheva V. V., Apyari V. V., Kochuk T. V., Dmitrienko S. G. Magnetic adsorbents based on iron oxide nanoparticles for the extraction and preconcentration of organic compounds. J. Analyt. Chem., 2016, vol. 71, no. 4, pp. 339-356. DOI: https://doi.org/10.7868/S0044450216040071
- Dzhenloda R. K., Petrov D. G., Shkinev V. M., Spivakov B. Y. DNA recovery from environmental sampleson suspension columns under a combined action of ultrasound and magnetic fi elds followed by polymerasechain reaction detection. Mend. Comm., 2017, vol. 27, no. 3, pp. 302-303. DOI: https://doi.org/10.1016/j.mencom.2017.05.029
- Egunova O. R., Shtykov S. N. Preconcentration of some fluoroquinolone antibiotics by magnetic solid-phase extraction using magnetite nanoparticles. Sorption and Chromatography Processes, 2018, vol. 18, no. 6, pp. 825-835 (in Russian). DOI: https://doi.org/10.17308/sorpchrom.2018.18/610
- Pryazhnikova D. V., Kiseleva M. S., Rubrakova I. V. Magnetik surface-modified nanosized sorbents for MSPE-HPLC-UV determination in natural waters. Analytics and Control, 2015, vol. 19, no. 3, pp. 220-229 (in Russian). DOI: http://dx.doi.org/10.15826/analitika.2015.19.3.006
- Tolmacheva V. V., Apyari V. V., Ibragimova B. N., Kochuk E. V., Dmitrienko S. G., Zolotov Y. A. A polymeric magnetic adsorbent based on Fe3O4 nanoparticles and hypercrosslinked polystyrene for the preconcentration of tetracycline antibiotics. Journal of Analytical Chemistry, 2015, vol. 70, no. 11, pp. 1149-1157. DOI: https://doi.org/10.7868/S004445021511016X
- Sadov A. A., Sumina E. G., Sorokina O. N., Uglanova V. Z. Khromatograficheskoye opredeleniye mel’doniya i karnitina metodom T·SKH s vodno-organicheskimi i mitsellyarnymi podvizhnymi fazami [Chromatographic determination of Meldonium and carnitine by TLC with water-organic and micellar mobile phases]. Sovremennyye problemy teoreticheskoy i eksperimental’noy khimii: mezhvuz. sb. nauch. tr. XII Vseros. konf. molodykh uchenykh s mezhdunar. uchastiyem (1-2 oktyabrya 2017, Saratov) [Modern problems of theoretical and experimental chemistry. Intercollegiate collection of scientifi c papers of the XII all-Russian conference of young scientists with international participation]. Saratov, Saratovskiy istochnik Publ., 2017, pp. 103-104 (in Russian).
- Egunova O. R., Reshetnikova I. S., German S. V., Kazimirova K. O., Khabibullin V. R., Zhelobitskaya E. A., Shtykov S. N. Sorption-fl uorimetric Determination of Enrofl oxacin with Magnetite Nanoparticles Modifi ed by Polyethylenimin. Izv. Saratov Univ. (N. S.), Ser. Chemistry. Biology. Ecology, 2016, vol. 16, iss. 1, pp. 48-52 (in Russian). DOI: https://doi.org/10.18500/1816-9775-2016-16-1-48-52
- Egunova O. R., Reshetnikova I. S., Kazimirova K. O., Shtykov S. N. Magnetic solid-phase extraction and fl uoremitrichesky defi nition of some ftorkhinolon. Journal of Analytical Chemistry, 2016, vol. 75, no. 1, pp. 31-42 (in Russian). DOI: https://doi.org/10.31857/S0044450220010065
- Lurye Yu. Yu. Spravochnik po analiticheskoy khimii [Handbook of analytical chemistry]. Мoscow, Khimiya Publ., 1971. 456 p. (in Russian).