Izvestiya of Saratov University.

Chemistry. Biology. Ecology

ISSN 1816-9775 (Print)
ISSN 2541-8971 (Online)
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Language: 
Russian
Heading: 
Chemistry
Article type: 
Article
UDC: 
543.645:543.054:543.544.943.3
DOI: 
10.18500/1816-9775-2020-20-4-378-386

Chromatographical Analysis of Meldonium with Preliminary Concentration by Solid-Phase Extraction on Magnetite Nanoparticles

Autors: 
Sumina Elena G., Saratov State University
Reshetnikova Irina Sergeevna, Saratov State University
Uglanova Varsenia Z., Saratov State University
Yurasov Nikolay Alexandrovich, Saratov State University
Abstract: 

A comprehensive method for meldonium analysis, based on a combination of magnetic solid-phase concentration of the analyte on magnetite nanoparticles modified with cetylammonium bromide (CTAB) cations and its subsequent analysis by thin-layer chromatography (TLC) were developed in the process of study. The influence of a number of factors on meldonium sorption was studied, namely: pH, sorbent mass, sorption and mixing times; optimal extraction conditions were determined. It was found that quantitative sorption proceeded in 20 min with a meldonium extraction degree of 88%. Ethanol was found to be an eluent to desorb the analyte by 95%. The calibration dependences plotted with and without concentration of meldonium were compared. It was established that the use of concentration on two mg of CTAB-modified nanomagnetite reduces the lower limit of the analyzed contents of meldonium in 25 ml of solution by 5 times.

Key words: 
meldonium
magnetic solid-phase extraction
magnetite
nanoparticles
thin-layer chromatography
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Received: 
30.11.2020