Izvestiya of Saratov University.

Chemistry. Biology. Ecology

ISSN 1816-9775 (Print)
ISSN 2541-8971 (Online)

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Evaluation of the secondary structure of poly-?-glutamic acid produced by Bacillus subtilis EGP5QL12 by circular dichroism spectroscopy method

Chernykh Marina Vladimirovna, Saratov State University
Ibrahim Ibrahim M., Saratov State University
Grinev Vyacheslav Sergeevich, Institute of Biochemistry and Physiology of Plants and Microorganisms of the Russian Academy of Sciences
Sigida Elena Nikolaevna, Institute of Biochemistry and Physiology of Plants and Microorganisms of the Russian Academy of Sciences
Fedonenko Yulia P., Institute of Biochemistry and Physiology of Plants and Microorganisms of the Russian Academy of Sciences
Konnova Svetlana Anatolyevna, Saratov State University

An extracellular polymer was isolated from the culture liquid of Bacillus subtilis EGP5QL12 with the yield of 5,6 g/L. On the basis of the data of thin layer chromatography, colorimetric analyses and FTIR spectroscopy, it was established that the polymer is poly-?glutamic acid (PGA). PGA is widely used in medicine, cosmetology and the food industry due to its ability to bind water and metal ions. To assess the biotechnological potential of the isolated polymer and predict the possibilities of its application in various fields of the national economy, it is necessary to analyze the characteristic spectral features that make it possible to establish its secondary structure. The isolated PGA preparation was analyzed by circular dichroism spectroscopy at various pH values. According to the results of this study, it was found that the polymer forms predominantly ?-structures with a low proportion of irregular structures and ?-helices, which gives it a high potential for creating hydrogels and composite materials.

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