The article presents the results of a comparative analysis of the distribution of plant species belonging to the families Typhaceae, Potamogetonaceae, Ruppiaceae, Juncaginaceae, Alismataceae, Butomaceae, Hydrocharitaceae in the Lower Volga region based on the materials of the herbarium of the Saratov State University named after N. G. Chernyshevsky (SARAT). Species of the families Typhaceae, Potamogetonaceae, Ruppiaceae, Juncaginaceae, Alismataceae, Butomaceae, Hydrocharitaceae are confi ned to coastal-aquatic and aquatic habitats vulnerable to anthropogenic factors, especially in the steppe zone. Analysis of the distribution of these species will help determine the methods of protecting such biotopes. During the entire existence of the herbarium of the Saratov State University named after N.G. Chernyshevsky (SARAT), a signifi cant amount of valuable material has been collected, which is currently being processed and systematized. To clarify the distribution of species, the data from the summary of the “Lower Volga Region Flora” and the Saratov State University Herbarium (SARAT) were analyzed, since only part of the herbarium data was used when writing the fi rst volume. The Saratov State University Herbarium (SARAT) registered 23 species of Potamogetonaceae, nine belong to Hydrocharitaceae, six to Alismataceae, fi ve to Typhaceae, two to Juncaginaceae, one each of Ruppiaceae and Butomaceae out of 60 species listed in the literature for the Lower Volga region. The most numerous is the Potamogetonaceae family (787 herbarium specimens), followed in descending order by Alismataceae (366), Hydrocharitaceae (243), Typhaceae (155), Butomaceae (121), Juncaginaceae (90), and Ruppiaceae (7). The collection currently contains 1,769 herbarium specimens belonging to the studied families, which were collected from 1902 to 2021 in the Lower Volga region. The largest number of specimens were herbarized in the Saratov region (1,260 herbarium specimens), followed by the Astrakhan region (262) and the Volgograd region (179). As a result of a comparative analysis for 37 species, the understanding of their distribution in the studied territory was expanded.