In the rivers of the Yenisei basin, benthic invertebrates play a major role in feeding most valuable Siberian fish species. This report presents advanced material on zoobenthos of the river Kan (one of the major tributaries of the Yenisei in its middle course). Litho-psammo-rheophilic biocenosis of benthic invertebrates was the most developed, 99 species and forms were recorded there. The total number of zoobenthos was formed from mayflies, caddisflies and chironomids; biomass – from caddisflies and mayflies.