Based on the Voronezh ANC named after V. V. Dokuchaev of the Department of Genetics and Immunity, electrophoretic studies were conducted, based on detailed study and analysis of polymorphism of prolamins of 5 varieties of winter wheat and their conjugation with economic and valuable features. Studies were conducted in 2022–2024 in the conditions of the southeast of the Central Black Earth Region. In the process, gliadin biotypes were identifi ed by electrophoresis in 6.5% polyacrylamide gel.