The article presents the results of a study of the vitality structure of four Delphinium litwinowii Sambuk coenopopulations from the Penza, Tambov and Voronezh regions in the period from 2017 to 2018. The most northern coenopopulation from Penza region in 2017 showed the strongest vitality, while the weakest was the Voronezh’ coenopopula- tion, located to the south, near the village of Vladimirovka.