Izvestiya of Saratov University.
ISSN 1816-9775 (Print)
ISSN 2541-8971 (Online)


Optimization of Cultivation Conditions of Azotobacter Vinelandii D-08 to Increase the Yield of Exopolysaccharide

In current research the optimization of culture conditions Azotobacter vinelandii D-08 on nutrient mediums with different content of molasses – 20, 30, 40 and 50% (by weight) was conducted. It is established that the maximum accumulation of polysaccharides was 13,78 g/l and it was observed at growth of the strain on the medium containing 50% molasses for 72 hours. The results of the method of gel permeation chromatography showed that the obtained polysaccharide has a molecular weight of 0.93532 kDa and 312.5014 kDa.

Preparation and Identification of the Molecular Properties of Alginate Synthesized by the Cultivation of Azotobacter vinelandii D-05

It is known that bacterial alginates are widely used in biomedicine as carriers in the immobilization of cells, enzymes and drugs. Their functional properties depend on their monomeric composition and molecular weight and vary depending on the source and the cultivation conditions. It was established that molasses (waste of sugar beet production) can be used as an effective and cheap source of nutrition for the growth of the bacterium Azotobacter vinelandii strain D-05 and the production of alginate.