DNA

Unlike antibiotics and heavy metals, nucleic acids exist in the aquatic environment as a part of prokaryotic and eukaryotic microorganisms (bacteria, fungi, etc.) rather than in a free form. In this regard, the most important primary stage of sample preparation of an object for the quantitative analysis of DNA and RNA in natural and wastewaters includes membrane ultrafi ltration of an aqueous sample, followed by its sorption preconcentration on a solid phase carrier.

The aim of the research is to determine the phylogenetic connections of scorpion representatives of the genus Mesobuthus living in the Lower Volga region and their comparison with other taxa (subspecies and species of this genus). For this, scorpions were collected on the territory of the Lower Volga region. The nucleotide sequences of the collected specimens were deciphered and compared with the sequences of species of the genus Mesobuthus from other regions of the Palearctic. The phylogenetic connections of the investigated objects are determined.