Izvestiya of Saratov University.

Chemistry. Biology. Ecology

ISSN 1816-9775 (Print)
ISSN 2541-8971 (Online)

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Bolshakov D. S., Shogah Z., Amelin V. G. Smartphone use in solid-phase fl uorimetric determination of some polypeptide antibiotics in medicinal preparations. Izvestiya of Saratov University. Chemistry. Biology. Ecology, 2023, vol. 23, iss. 3, pp. 252-261. DOI: 10.18500/1816-9775-2023-23-3-252-261, EDN: ZPAUBY

This is an open access article distributed under the terms of Creative Commons Attribution 4.0 International License (CC-BY 4.0).
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Language: 
Russian
Heading: 
Chemistry
Article type: 
Article
UDC: 
543.068.8:543.426:543.062:577.182.99
DOI: 
10.18500/1816-9775-2023-23-3-252-261
EDN: 
ZPAUBY

Smartphone use in solid-phase fl uorimetric determination of some polypeptide antibiotics in medicinal preparations

Autors: 
Bolshakov D. S., GENERIUM
Shogah Zen Аlabden Chalawi , Vladimir State University named after Alexander and Nikolay Stoletovs
Amelin V. G., Vladimir State University named after Alexander and Nikolay Stoletovs
Abstract: 

The analysis of semi-products at diff erent stages of production and fi nished medicinal products is an integral part of an eff ective quality assurance system for pharmaceutical production. The traditional methods used in the pharmaceutical fi eld, such as capillary electrophoresis, gas and liquid chromatography, are quite versatile and eff ective. However, they are distinguished by the high cost of instrumentation and component materials, the need to use high-purity solvents, the lack of mobility and the possibility of in situ studies. Such shortcomings can be overcome by using the digital colorimetry method. Its signifi cant popularization has been facilitated by the development of portable and personal electronic devices, among which the smartphone can be distinguished. The aim of the work was to develop a fast and simple method for the determination of a number of polypeptide antibiotics in drugs by digital colorimetry using a smartphone as a color recording device. To implement the solid phase fl uorimetric determination of polypeptide antibiotics, HPTLC plates based on silica gel with an aluminum substrate were used as a matrix. Under these conditions, the fl uorescence of actinomycin D, virginiamycin M1, virginiamycin S1, and novobiocin was observed when exposed to monochromatic radiation in the UV part of the spectrum (365 nm). The measurement of fl uorescence intensity on the surface of the matrices was carried out using a smartphone. Calibration characteristics were constructed for the determination of polypeptide antibiotics in the concentration range of 32–500 μg/mL. The method for assessing the quality of fi nished drugs was tested on commercial products “Piostacin”, “Cosmegen”, “Dactinomycin” purchased in retail chains. The relative standard deviation of the analysis results does not exceed 0.05. The duration of the analysis was 10–15 min. Based on the results of the studies, the paper presents a method for determining some antibiotics of the polypeptide series by the intrinsic fl uorescence of deposited solutions on a solid substrate during treatment with monochromatic UV radiation. The experimental delivery conditions used allow the analysis of drugs based on actinomycin D, virginiamycin M1, virginiamycin S1, and novobiocin.

Key words: 
polypeptide antibiotics
digital colorimetry
solid phase fl uorimetry
smartphone
drugs
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Received: 
12.04.2023
Accepted: 
23.05.2023
Published: 
29.09.2023
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