Izvestiya of Saratov University.

Chemistry. Biology. Ecology

ISSN 1816-9775 (Print)
ISSN 2541-8971 (Online)


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Mokeev D. I., Telesheva E. M., Volokhina I. V., Yevstigneyeva S. S., Pylaev T. E., Petrova L. P., Filip’echeva Y. A., Shelud’ko A. V. Genomic rearrangements aff ect the resistance of biofi lms of soil bacteria Azospirillum brasilense to abiotic stress. Izvestiya of Saratov University. Chemistry. Biology. Ecology, 2023, vol. 23, iss. 4, pp. 426-436. DOI: 10.18500/1816-9775-2023-23-4-426-436, EDN: JPBABO

This is an open access article distributed under the terms of Creative Commons Attribution 4.0 International License (CC-BY 4.0).
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Russian
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579.26:574.23
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JPBABO

Genomic rearrangements aff ect the resistance of biofi lms of soil bacteria Azospirillum brasilense to abiotic stress

Autors: 
Mokeev Dmitriy I., Institute of Biochemistry and Physiology of Plants and Microorganisms – Subdivision of the Federal State Budgetary Research Institution Saratov Federal Scientifi c Centre of the Russian Academy of Sciences (IBPPM RAS)
Telesheva Elizaveta M., Institute of Biochemistry and Physiology of Plants and Microorganisms – Subdivision of the Federal State Budgetary Research Institution Saratov Federal Scientifi c Centre of the Russian Academy of Sciences (IBPPM RAS)
Volokhina Irina V., Institute of Biochemistry and Physiology of Plants and Microorganisms – Subdivision of the Federal State Budgetary Research Institution Saratov Federal Scientifi c Centre of the Russian Academy of Sciences (IBPPM RAS)
Yevstigneyeva Stella S., Institute of Biochemistry and Physiology of Plants and Microorganisms of the Russian Academy of Sciences
Pylaev Timofey E., Institute of Biochemistry and Physiology of Plants and Microorganisms of the Russian Academy of Sciences
Petrova Liliya P., Institute of Biochemistry and Physiology of Plants and Microorganisms of the Russian Academy of Sciences
Filip’echeva Yulia A., Institute of Biochemistry and Physiology of Plants and Microorganisms of the Russian Academy of Sciences
Shelud’ko Andrei V., Institute of Biochemistry and Physiology of Plants and Microorganisms of the Russian Academy of Sciences
Abstract: 

The bacteria Azospirillum brasilense, used as biofertilizers, have a signifi cant positive eff ect on the growth and development of plants. The genome of the strain A. brasilense Sp7 is represented by a chromosome and numerous plasmids with molecular weight of 90, 115, and over 300 MDa. Genomic rearrangements that cause changes in the “plasmid profi le” can contribute to the formation of subpopulations or phenotypic variants in a bacterial population. There is little data on the role of such rearrangements in the adaptation of A. brasilenseto dynamic environmental conditions. The ability of azospirilla to form biofi lms also has a determined signifi cance for the successful functioning of the plant-microbial association and the resistance of bacteria and plants to various abiotic stresses. The purpose of this work consisted of the analysis of the genomic rearrangements in spontaneous derivatives of A. brasilense Sp7 and the assessment of the resistance of their biofi lms to drying, water stress and oxidative stress. PCR analysis to detect changes in the structure of genomic DNA was performed using primers corresponding to known conservative motifs in repetitive bacterial nucleotide sequences. The relative amount of the biofi lm biomass was assessed by measuring the crystal violet A540 desorbed after staining. The level of relative respiratory activity of cells in biofi lms was determined by the fl uorometric resazurin test. The non-penetrating osmotic agent PEG 6000 was used to create the osmotic/water stress model. It was shown that rearrangements in genomic DNA contribute to the formation of stable phenotypic variants of the Sp7 strain, which form biofi lms in diff erent ways under water stress conditions. A derived strain of A. brasilense Sp7.8, the biofi lm population of which is more resistant to water stress compared to the parent strain was selected.

Reference: 
  1. Fibach-Paldi S., Burdman S., Okon Y. Key physiological properties contributing to rhizosphere adaptation and plant growth promoting abilities of Azospirillum brasilense // FEMS Microbiol. Lett. 2012. Vol. 326. P. 99–108. https://doi.org/10.1111/j.1574-6968.2011.02407.x
  2. Fukami J., Cerezini P., Hungria M. Azospirillum: benefi ts that go far beyond biological nitrogen fi xation // AMB Expr. 2018. Vol. 8. P. 73–85. https://doi.org/10.1186/s13568-018-0608-1
  3. Lipa P., Janczarek M. Phosphorylation systems in symbiotic nitrogen-fi xing bacteria and their role in bacterial adaptation to various environmental stresses // PeerJ. 2020. Feb11 : 8 : e8466. https://doi.org/10.7717/peerj.8466
  4. Ansari F. A., Jabeen M., Ahmad I. Pseudomonas azotoformans FAP5, a novel biofi lm-forming PGPR strain, alleviates drought stress in wheat plant // Int. J. Environ. Sci. Technol. 2021. Vol. 18. P. 3855–3870. https://doi. org/10.1007/s13762-020-03045-9
  5. Vurukonda S. S. K. P., Sandhya V., Shrivastava M., Ali S. K. Z. Enhancement of drought stress tolerance in crops by plant growth promoting rhizobacteria // Microbiol.Res. 2016. Vol. 184. P. 13–24. https://doi. org/10.1016/j.micres.2015.12.003
  6. Hsiao T. C. Plant responses to water stress // Ann. Rev. Plant Physiol. 1973. Vol. 24. P. 519–570. https://doi.org/10.1146/annurev.pp.24.060173.002511
  7. Bogino P. C., Oliva M. M., Sorroche F. G., Giordano W. The role of bacterial biofi lms and surface components in plant-bacterial associations // Int. J. Mol. Sci. 2013. Vol. 14. P. 15838–15859. https://doi.org/10.3390/ijms140815838
  8. Lerner A., Valverde A., Castro-Sowinski S., Lerner H., Okon Y., Burdman S. Phenotypic variation in Azospirillum brasilense exposed to starvation // Environ. Microbiol. Rep. 2010. Vol. 2. P. 577–586. https://doi.org/10.1111/ j.1758-2229.2010.00149.x
  9. Volfson V., Fibach-Paldi Sh., Paulucci N. S., Dardanelli M., Matan O., Burdman S., Okon Y. Phenotypic variation in Azospirillum brasilense Sp7 does not infl uence plant growth promotion effects // Soil Biology and Biochemistry. 2013. Vol. 67. P. 255–262. https://doi.org/10.1016/j.soilbio.2013.09.008
  10. Petrova L. P., Borisov I. V., Katsy E. I. Plasmid rearrangements in Azospirillum brasilense // Microbiology (Moscow). 2005. Vol. 74, № 4. P. 495–497. https://doi.org/10.1007/s1102100500948
  11. Petrova L. P., Shelud’ko A. V., Katsy E. I. Plasmid rearrangements and alterations in Azospirillum brasilense biofilm formation // Microbiology (Moscow). 2010. Vol. 79, № 1. P. 121–124. https://doi.org/10.1134/ S00226261710010169
  12. Katsy E. I., Petrova L. P. Genome rearrangements in Azospirillum brasilense Sp7 with the involvement of the plasmid pRhico and the prophage ΦAb-Cd // Russ. J. Genet. 2015. Vol. 51, № 132. P. 1165–117. https://doi. org/10.1134/S1022795415110095
  13. Shelud’ko A. V., Mokeev D. I., Evstigneeva S. S., Filip’echeva Yu. A., Burov A. M., Petrova L. P., Ponomareva E. G., Katsy E. I. Cell ultrastructure in biofi lms of Azospirillum brasilense // Microbiology. 2020. Vol. 89, № 1. P. 50–63. https://doi.org/10.1134/S0026261720010142
  14. Flemming H.-C., Wingender J. The biofi lm matrix // Nat. Rev. Microbiol. 2010. Vol. 8, № 9. P. 623–633. https://doi.org/10.1038/nrmicro2415
  15. Ramírez-Mata A., López-Lara L. I., Xiqui-Vázquez L., Jijón-Moreno S., Romero-Osorio A., Baca B. E. The cyclic-di-GMP diguanylate cyclase CdgA has a role in biofi lm formation and exopolysaccharide production in Azospirillum brasilense // Res. Microbiol. 2016. Vol. 167. P. 190–201. https://doi.org/10.1016/j.resmic.2015.12.004
  16. Wang D., Xu A., Elmerich C., Ma L. Z. Biofi lm formation enables free-living nitrogen-fi xing rhizobacteria to fi x nitrogen under aerobic conditions // ISME J. 2017. Vol. 11. P. 1602–1613. https://doi.org/10.1038/ismej. 2017.30
  17. Shelud’ko A. V., Filip’echeva Yu. A., Telesheva E. M., Burov A. M., Evstigneeva S. S., Burygin G. L., Petrova L. P. Characterization of carbohydrate-containing components of Azospirillum brasilense Sp245 biofi lms // Microbiology. 2018. Vol. 87, № 5. P. 610–620. https://doi.org/10.1134/S0026261718050156.
  18. Wisniewski-Dyé F., Vial L. Phase and antigenic variation mediated by genome modifi cations // Antonie van Leeuwenhoek J. Microbiol. 2008. Vol. 94. P. 493–515. https://doi.org/10.1007/s10482-008-9267-6
  19. Versalovic J., Koeuth T., Lupski R. Distribution of repetitive DNA sequences in eubacteria and application to fi nerpriting of bacterial genomes // Nucleic Acids Res. 1991. Vol. 19, № 24. P. 6823–6831. https://doi.org/10.1093/ nar/19.24.6823
  20. Fancelli S., Castaldini M., Ceccherini M. T., Di Serio C., Fani R., Gallori E., Marangolo M., Miclaus N., Bazzicalupo M. Use of random amplifi ed polymorphic DNA markers for the detection of Azospirillum strains in soil microcosms // Appl. Microbiol. Biotechnol. 1998. Vol. 49, № 2. P. 221–225. https://doi.org/10.1007/s002530051162
  21. Tarrand J. J., Krieg N. R., Döbereiner J. A taxonomic study of the Spirillum lipoferum group with description of a new genus, Azospirillum gen. nov. and two species, Azospirillum lipoferum (Beijerinck) comb. nov. and Azospirillum braslense sp. nov. // Can. J. Microbiol. 1978. Vol. 24, № 8. P. 967–980. https://doi.org/10.1139/m78-160
  22. Eskew D. L, Focht D. D., Ting L. P. Nitrogen fi xation, denitrification and pleomorphic growth in a highly pigmented Spirillum lipoferum // Appl. Environ. Microbiol. 1977. Vol. 34. P. 582–585. https://doi.org/10.1128/aem.34.5.582-585.1977
  23. Döbereiner J., Day J. M. Associative symbiosis in tropical grass: Characterization of microorganisms and dinitrogen fi xing sites // Symposium on Nitrogen Fixation / eds. W. E. Newton, C. J. Nijmans. Pullman : Washington State University Press, 1976. P. 518–538.
  24. O’Toole G. A., Kolter R. Initiation of biofi lm formation in Pseudomonas fl uorescens WCS365 proceeds via multiple, convergent signalling pathways: A genetic analysis // Mol. Microbiol. 1998. Vol. 28, № 3. P. 449–461. https://doi.org/10.1046/j.1365-2958.1998.00797.x
  25. Chutia J., Borah S. P. Water stress effects on leaf growth and chlorophyll content but not the grain yield in traditional rice (Oryza sativa Linn.) genotypes of Assam, India: II. Protein and proline status in seedlings under PEG induced water stress // Am. J. Plant Sci. 2012. Vol. 3. P. 971–980. http://dx.doi.org/10.4236/ajps.2012.37115
  26. Malinich E. A., Bauer C. E. The plant growth promoting bacterium Azospirillum brasilense is vertically transmitted in Phaseolus vulgaris (common bean) // Symbiosis. 2018. Vol. 76, № 2. P. 97–108. https://doi.org/10.1007/s13199-018-0539-2
  27. Schloter M., Hartmann A. Endophytic and surface colonization of wheat roots (Triticum aestivum) by different Azospirillum brasilense strains studied with strain-specifi c monoclonal antibodies // Symbiosis. 1998. Vol. 25. P. 159–179. 
  28. Pradedova E. V., Isheeva O. D., Salyaev R. K. Classification of the antioxidant defense system as the ground for reasonable organization of experimental studies of the oxidative stress in plants // Russ. J. Plant. Physiol. 2011. Vol. 58. P. 210–217. https://doi.org/10.1134/ S1021443711020166
  29. Notununu I., Moleleki L., Roopnarain A., Adeleke R. Effects of plant growth-promoting rhizobacteria on the molecular responses of maize under drought and heat stresses: A review // Pedosphere. 2022. Vol. 32. P. 90–106. https://doi.org/10.1016/S1002- 0160(21)60051-6
Received: 
01.06.2023
Accepted: 
01.07.2023
Published: 
25.12.2023
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