Izvestiya of Saratov University.

Chemistry. Biology. Ecology

ISSN 1816-9775 (Print)
ISSN 2541-8971 (Online)


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Abalymov A. A., Lomova M. V., Novoselova M. V., Gorin D. A. Evaluation of polymeric submicron microcapsule accumulation in cellular and intercellular space of 3D spheroids. Izvestiya of Saratov University. Chemistry. Biology. Ecology, 2024, vol. 24, iss. 2, pp. 163-171. DOI: 10.18500/1816-9775-2024-24-2-163-171, EDN: BWUINF

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577.359
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BWUINF

Evaluation of polymeric submicron microcapsule accumulation in cellular and intercellular space of 3D spheroids

Autors: 
Abalymov A. A., Saratov State University
Lomova Maria V., Saratov State University
Novoselova Marina V., Skolkovo Institute of Science and Technology
Gorin Dmitriy A., Saratov State University
Abstract: 

Multicellular spheroids are three-dimensional in vitro models of organs and tissues. Multicellular spheroids have gained great interest in the fi eld of biotechnology as they are reproducible and mimic real organs and tissues so can be used as test systems for new forms of drugs, allowing minimizing the use of in vivo animal models. Flow cytometry was used to describe the accumulation of submicron polymeric microcapsules in cellular spheroids, which allowed us to identify the main aspects of drug carrier-cell interactions within the spheroid. Spheroids were generated using 4T1 mouse breast cancer cells and healthy L929 mouse fi broblast cells. The arrangement of microcapsules with 300, 500, 1000 nm diameter with biocompatible shells in cell spheroids was evaluated in their intercellular and intracellular spaces.

Reference: 
  1. Voronin D. V., Abalymov A. A., Svenskaya Y. I., Lomova M. V. Key points in remote – controlled drug delivery: From the carrier design to clinical trials. Int. J. Mol. Sci., 2021, vol. 22, article number 9149. https://doi.org/10.3390/ijms22179149
  2. Saveleva M. S., Eftekhari K., Abalymov A., Douglas T. E. L., Volodkin D., Parakhonskiy B. V., Skirtach A. G. Hierarchy of hybrid materials-the place of inorganics-in-organics in it, their composition and applications. Front. Chem., 2019, vol. 7, pp. 1–21. https://doi.org/10.3389/fchem.2019.00179
  3. De Koker S., Hoogenboom R., De Geest B. G. Polymeric multilayer capsules for drug delivery. Chem. Soc. Rev., 2012, vol. 41, article number 2867. https://doi.org/10.1039/c2cs15296g
  4. Tong W., Dong W., Gao C., Möhwald H. Charge-controlled permeability of polyelectrolyte microcapsules. J. Phys. Chem. B, 2005, vol. 109, pp. 13159–13165. https://doi.org/10.1021/jp0511092
  5. Van der Meeren L., Li J., Konrad M., Skirtach A. G., Volodkin D., Parakhonskiy B. V. Temperature window for encapsulation of an enzyme into thermally shrunk, CaCO3 templated polyelectrolyte multilayer capsules. Macromol. Biosci., 2020, vol. 20. https://doi.org/10.1002/mabi.202000081
  6. Ejima H., Yanai N., Best J. P., Sindoro M., Granick S., Caruso F. Near–incompressible faceted polymer microcapsules from metal – organic framework templates. Adv. Mater., 2013, vol. 25, pp. 5767–5771. https://doi.org/10.1002/adma.201302442
  7. Li J., Khalenkow D., Volodkin D., Lapanje A., Skirtach A. G., Parakhonskiy B. V. Surface enhanced Raman scattering (SERS)-active bacterial detection by Layer-by-Layer (LbL) assembly all-nanoparticle microcapsules. Colloids https://doi.org/10.1016/j.colsurfa.2022.129547
  8. Vidiasheva I. V., Abalymov A. A., Kurochkin M. A., Mayorova O. A., Lomova M. V., German S. V., Khalenkow D. N., Zharkov M. N., Gorin D. A., Skirtach A. G. Transfer of cells with uptaken nanocomposite, magnetite-nanoparticle functionalized capsules with electromagnetic tweezers. Biomater. Sci., 2018, vol. 6, pp. 2219–2229. https://doi.org/10.1039/c8bm00479j
  9. Demina P. A., Abalymov A. A., Voronin D. V., Sadovnikov A. V., Lomova M. V. Highly-magnetic mineral protein-tannin vehicles with anti-breast cancer activity. Mater. Chem. Front., 2021, vol. 5, pp. 2007–2018. https://doi.org/10.1039/d0qm00732c
  10. Lengert E., Saveleva M., Abalymov A., Atkin V., Wuytens P. C., Kamyshinsky R., Vasiliev A. L., Gorin D. A., Sukhorukov G. B., Skirtach A. G. Silver alginate hydrogel micro- and nanocontainers for theranostics: synthesis, encapsulation, remote release, and detection. ACS Appl. Mater. Interfaces, 2017, vol. 9, pp. 21949–21958. https://doi.org/10.1021/acsami.7b08147
  11. Abalymov A. A., Verkhovskii R. A., Novoselova M. V., Parakhonskiy B. V., Gorin D. A., Yashchenok A. M., Sukhorukov G. B. Live-cell imaging by confocal raman and fluorescence microscopy recognizes the crystal structure of calcium carbonate particles in hela cells. Biotechnol. J., 2018, vol. 13, article number 1800071. https://doi.org/10.1002/biot.201800071
  12. German S. V., Abalymov A. A., Kurochkin M. A., Kan Y., Gorin D. A., Novoselova M. V. Plug-and-play lymph node-on-chip: secondary tumor modeling by the combination of cell spheroid, collagen sponge and T-сells. Int. J. Mol. Sci., 2023, vol. 24, article number 3183. https://doi.org/10.3390/ijms24043183
  13. Anisimov R. A., Gorin D. A., Abalymov A. A. 3D Cell spheroids as a tool for evaluating the effectiveness of carbon nanotubes as a drug delivery and photothermal therapy agents. J. Carbon Res. C, 2022, vol. 8, pp. 56. https://doi.org/10.3390/c8040056
  14. Lu H., Stenzel M. H. Multicellular tumor spheroids (MCTS) as a 3D in vitro evaluation tool of nanoparticles. Small, 2018, vol. 14, article number 1702858. https://doi.org/10.1002/smll.201702858
  15. Moshksayan K., Kashaninejad N., Warkiani M. E., Lock J. G., Moghadas H., Firoozabadi B., Saidi M. S., Nguyen N. T. Spheroids-on-a-chip: Recent advances and design considerations in microfl uidic platforms for spheroid formation and culture. Sensors Actuators, B Chem., 2018, vol. 263, pp. 151–176. https://doi.org/10.1016/j.snb.2018.01.223
  16. Parakhonskiy B., Zyuzin M. V., Yashchenok A., Carregal-Romero S., Rejman J., Möhwald H., Parak W. J., Skirtach A. G. The infl uence of the size and aspect ratio of anisotropic, porous CaCO3 particles on their uptake by cells. J. Nanobiotechnology, 2015, vol. 13, iss. 1, pp. 53. https://doi.org/10.1186/s12951-015-0111-7 
Received: 
18.11.2023
Accepted: 
28.11.2023
Published: 
31.05.2024
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