Izvestiya of Saratov University.

Chemistry. Biology. Ecology

ISSN 1816-9775 (Print)
ISSN 2541-8971 (Online)

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Abalymov A. A., Lomova M. V., Novoselova M. V., Gorin D. A. Evaluation of polymeric submicron microcapsule accumulation in cellular and intercellular space of 3D spheroids. Izvestiya of Saratov University. Chemistry. Biology. Ecology, 2024, vol. 24, iss. 2, pp. 163-171. DOI: 10.18500/1816-9775-2024-24-2-163-171, EDN: BWUINF

This is an open access article distributed under the terms of Creative Commons Attribution 4.0 International License (CC-BY 4.0).
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Language: 
Russian
Heading: 
Biology
Article type: 
Article
UDC: 
577.359
DOI: 
10.18500/1816-9775-2024-24-2-163-171
EDN: 
BWUINF

Evaluation of polymeric submicron microcapsule accumulation in cellular and intercellular space of 3D spheroids

Autors: 
Abalymov A. A., Saratov State University
Lomova Maria V., Saratov State University
Novoselova Marina V., Skolkovo Institute of Science and Technology
Gorin Dmitriy A., Saratov State University
Abstract: 

Multicellular spheroids are three-dimensional in vitro models of organs and tissues. Multicellular spheroids have gained great interest in the fi eld of biotechnology as they are reproducible and mimic real organs and tissues so can be used as test systems for new forms of drugs, allowing minimizing the use of in vivo animal models. Flow cytometry was used to describe the accumulation of submicron polymeric microcapsules in cellular spheroids, which allowed us to identify the main aspects of drug carrier-cell interactions within the spheroid. Spheroids were generated using 4T1 mouse breast cancer cells and healthy L929 mouse fi broblast cells. The arrangement of microcapsules with 300, 500, 1000 nm diameter with biocompatible shells in cell spheroids was evaluated in their intercellular and intracellular spaces.

Key words: 
spheroids
fl ow cytometry
microcapsules
drug delivery
theranostics
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Received: 
18.11.2023
Accepted: 
28.11.2023
Published: 
31.05.2024
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