Key to the subfamilies of chironomids (Chironominae) based on the morphology of the male genitalia includes 16 genus and 45 species found in the Volga river floodplain lakes (Saratov region). For the six species of the four genera of the tribe Chironomini (Einfeldia sp.1, Chironomus sp. (Lobochironomus), Dicrotendipes sp.1, Dicrotendipes sp.2, Parachironomus sp., Polypedilum sp.) described morphological differences from the known and the most directly comparable species.