Izvestiya of Saratov University.
Chemistry. Biology. Ecology
ISSN 1816-9775 (Print)
ISSN 2541-8971 (Online)

пероксидаза хрена

Evaluation of activity of peroxidases contained in various plant sources

In this study, the decolorization reaction of aqueous solution bromophenol blue dye was carried out in the presence of hydrogen peroxide and peroxidase contained in the roots of horseradish (Armoracia rusticana), radish (Raphanus sativus var. radicula), yellow turnip (Brassica napobrassica), daikon (Raphanus sativus), black radish (Raphanus sativus ‘Niger’), green radish (Raphanus) or stalk of white cabbage (Brassica capitata).

Оценка активности пероксидаз, содержащихся в различных растительных источниках

В данной работе была проведена ферментативная деструкция красителя бромфенолового синего в присутствии пероксида водорода и пероксидазы, содержащейся в корнеплодах хрена обыкновенного (Armoracia rusticana), редиса (Raphanus sativus var. radicula), репы желтой (Brassica napobrassica), дайкона (Raphanus sativus), редьки черной (Raphanus sativus 'Niger'), редьки зеленой (Raphanus), либо кочерыжке капусты белокочанной (Brassica capitata).

Sensor Plate in Horseradish Peroxidase Determination Based on Oxidation of Triphenylamine-4-sulphonic Acid by Hydrogene Peroxide

The oxidation of triphenylamine-4-sulphonic acid (TPASA) by hydrogen peroxide in presence of horseradish peroxidase (HRP) in weak acid medium and after incorporation of TPASA in polymeric hydrogel matrix of polyurethane (type D4) was investigated by spectrophotometry. The stability of TPASA product enzymatic oxidation under variation of reaction condition (reagents concentration, acidity) was studied. The optimum condition was selected and the possibility of HRP detection in sensor plate was shown with limit of detection 0.3•10-4 U/mL.