The influence of coatings on the basis of Ag and Zn metal nanoparticles in a silica matrix in combination with ultraviolet (365 nm) radiation on Staphylococcus aureus 209 P bacteria was studied. It was shown that the investigated coatings without access to light inhibit the growth of microorganisms by 45% after 3 hours of incubation and 55–70% after 5 hours of incubation. Enhancement of antibacterial properties of nanocoatings was achieved by exposure to UV (365 nm) radiation.